Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
42
1
2019
12
31
MICROORGANISMS ASSOCIATED WITH DIABETIC FOOT ULCERS
1
7
62203
10.21608/bfsa.2019.62203
EN
Khaled Mohammed
Hassanin
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
Entsar Hamed
Ahmed
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
Mohammed Ahmed
Al-Mokhtar
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
Hend
Youssef
Al-Rajhy Liver Hospital, Assiut University, Assiut, Egypt
Journal Article
2019
02
26
Background: Non-traumatic lower limb amputation is the most common devastating complication of diabetes, primarily due to diabetic foot ulcers (DFU) and diabetic foot infections (DFI). DFIs are predominantly polymicrobial and multidrug-resistant (MDR) and results in treatment failure. Aims: The main objectives of the study are to identify the microorganisms associated with diabetic foot ulers. Methods: This was a prospective study at a tertiary care hospital. One hundred patients over the age of 18, having chronic diabetic foot ulcer, and attending the diabetic foot outpatient department were included. Samples of pus were collected from deep wounds and processed using standard techniques for culture Results: One hundred samples were processed and 82 yielded positive cultures. Staphylococcus aureus was the predominant organism, followed by Pseudomonas aeruginosa. Then E coli, Klebsiella and Proteus Conclusion: The organisms causing chronic diabetic foot ulcers were commonly Staphylococcus aureus followed by Pseudomonas aeruginosa
https://bpsa.journals.ekb.eg/article_62203_3dbdc14c57ee705f8a5afeac966012f2.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
42
1
2019
12
31
B (REG) CELLS IN HEPATITIS C VIRUS AND DIABETES
9
18
62254
10.21608/bfsa.2019.62254
EN
Khaled Mohammed
Hassanin
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
Omnia Hassan
Bakr
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
Helal Fouad
Hetta
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
Mohamed Omar
Abdel Malek
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
Fadwa
Mohammed
Assiut Center Administration, Assiut, Egypt
Journal Article
2019
04
04
Over the last decade it has become evident that in addition to producing antibody, B cells activate the immune system by producing cytokines and via antigen presentation. In addition, B cells also exhibit immunosuppressive functions via diverse regulatory mechanisms. This subset of B cells, known as regulatory B cells (B regs), contributes to the maintenance of tolerance, primarily via the production of IL-10. Studies in experimental animal models, as well as in patients with autoimmune diseases, have identified multiple B(reg) subsets exhibiting diverse mechanisms of immune suppression. In this review, we describe the different B(reg) subsets identified in humans, and their diverse mechanisms of suppression in HCV and diabetic patients. Aims: The main objectives of the study are to identify the role of B (reg) in the hepatitis C virus infected patients and diabetes. <br /> <br />
https://bpsa.journals.ekb.eg/article_62254_ab38dc85d165fe3ee7ad2e92445459c9.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
42
1
2019
12
31
HYPOGLYCEMIC AND HYPOLIPIDEMIC EFFECTS OF GINGER IMPROVE KIDNEY FUNCTION IN OBESE MALE RATS
19
29
62260
10.21608/bfsa.2019.62260
EN
Amira M.
El-Noweihi
Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Egypt
Nagla Taha
Elmelegy
Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Egypt
Sally M.
Bakar
Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Egypt
Shimaa
El-Nasser
Department of Biochemistry, Faculty of Pharmacy, South Valley University, Egypt
Journal Article
2019
04
18
Aside from being a social stigma, obesity is frequently associated with insulin resistance, in turn linked to development of type II diabetes, hypertension, hyperlipidemia, and atherosclerosis – the so-called metabolic syndrome.This study investigated the biochemical changes in serum urea and creatinine levels in obese male rats treated with aqueous ginger extract. Forty age-matched adult male wister rats (90-110) gm were divided into four groups of ten rats each: Group I, Control group; Group II: Obese group. Group III: Low ginger dose (200 mg/kg body weight) treated obese group; Group IV: High ginger dose (400 mg/kg body weight) treated obese group.The obese group exhibited hyperglycemia accompanied with increasing in serum levels of Triglycerides (TG), Low Density Lipoprotein Cholesterol (LDL-C), Total Cholesterol (TC) levels. On the other hand, there was a significant reduction in High Density Lipoprotein Cholesterol (HDL-C) level. Ginger was effective in lowering all previous mentioned biochemical parameters and HDL-C level was increased significantly. Serum urea and creatinine levels showed a significant increase in obese rats. Otherwise, obese rats treated with ginger at either dose revealed a significant decrease as compared to obese group. These results indicated that the hypoglycemic and hypolipidemic effects of aqueous ginger extract (200, 400 mg/kg/day) could ameliorate obesity related kidney dysfunction.
https://bpsa.journals.ekb.eg/article_62260_315899192f95205bca4fa213204a9fd0.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
42
1
2019
12
31
ENHANCEMENT OF LIPOSOMAL DRUG LOADING BY USING SUPERSATURATED DRUG SOLUTION
31
38
62263
10.21608/bfsa.2019.62263
EN
Hassan
Tamam
Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
Department of Industrial and Physical Pharmacy, Purdue University, 575 Stadium Mall Drive, West Lafayette, IN 47907, USA
Jelan A.
Abdel-Aleem
Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
Sayed I.
Abdelrahman
Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
Aly A.
Abdelrahman
Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
Yoon
Yeo
Department of Industrial and Physical Pharmacy, Purdue University, 575 Stadium Mall Drive, West Lafayette, IN 47907, USA
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
Journal Article
2019
04
24
Liposomes are used for systemic delivery of chemotherapeutic drugs to reduce their nonspecific side effects. Liposomes can encapsulate hydrophilic and lipophilic drugs in the water compartment and the lipid membrane, respectively. However, typical drug loading capacity of liposomes by passive loading method is less than 1%. The low drug loading efficiency is problematic because it necessitates the use of a large amount of carrier materials that may cause undesirable biological effects. To increase drug loading in liposomes, weusedsupersaturated drug solutionswith gemcitabine (GEM) and doxorubicin (Dox) as examples. The prepared liposomes showed higher drug loading compared with passive loading, maintainedstabilityand provided sustained drug release for 48 hrs.
https://bpsa.journals.ekb.eg/article_62263_b34aba8b016f8575307fc79565651858.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
42
1
2019
12
31
STEVIA IMPROVES THE ANTIHYPERGLYCEMIC EFFECT OF METFORMIN IN STREPTOZOTOCIN-INDUCED DIABETIC RATS: A NOVEL STRATEGY IN TYPE 2 DIABETES MELLITUS
39
50
62264
10.21608/bfsa.2019.62264
EN
Raafat A.
Abdel-Aal
Department of Pharmacology, Faculty of Medicine, Assiut University, Egypt
Mahran S.
Abdel-Rahman
Department of Pharmacology, Faculty of Medicine, Assiut University, Egypt
Laila
Ali
Department of Pharmacology, Faculty of Pharmacy, Assiut University, Egypt
Journal Article
2019
07
09
Diabetes mellitus is a major health problem that threatens the whole world. According to WHO reports, the prevalence of diabetic patients in egyptis expected to increase from 2,623,000 in 2000 to be 6,726,000 in 2030. Metformin is the first line drug for type 2 diabetes mellitus, which can be used alone or in combination with other drugs. However, the concomitant use of metformin with stevia needs more investigation to clarify the role of this combination as a new strategy in type 2 diabetes mellitus. Type 2 diabetes mellitus was induced in rats by i.p. injection of STZ and NA. Animals were divided into five groups, each contains 8 rats. Group I: negative control, group II: diabetic control received saline, group III: diabetic rats received 400 mg/kg/day stevia aqueous extract, group IV: diabetic rats received metformin 250 mg/kg/day, group V: diabetic rats received stevia 400 mg/kg/day + metformin 250 mg/kg/day. After 3 weeks blood samples were collected, animals were sacrificed and tissue samples were collected. Biochemical parameters including FBG, serum insulin, serum DPP-4, TC, TG, LDL, HDL, GSH and MDA were measured by colorimetric and ELISA methods. Both stevia and metformin significantly reduced FBG level. While serum insulin significantly increased. Serum DPP-4 was significantly reduced in all treated groups, concerning lipid profile, stevia and metformin significantly lowered TC, TG, LDLand increased HDL. Both stevia and metformin significantly decreased MDA and increased GSH compared to diabetic rats. In addition, stevia significantly improved the antidiabetic effects of metformin. Stevia has an antihyperglycemic effect and could increase the antidiabetic activity of metformin. DPP-4 attenuation, antioxidant and insulin-sensitizing effects may be involved in the antidiabetic action of stevia. Regarding lipid profile stevia showed hypolipidemic effect. <br />
https://bpsa.journals.ekb.eg/article_62264_c529f7c228660825f11e692e8174bcae.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
42
1
2019
12
31
DEVELOPMENT AND CHARACTERIZATION OF NANOSTRUCTURED LIPID CARRIERS FOR TRANSDERMAL DELIVERY OF MELOXICAM
51
62
62265
10.21608/bfsa.2019.62265
EN
Radwa
Radwan
Assiut International Center of Nanomedicine, Al-Rajhy Liver Hospital, Assiut University, Assiut, Egypt
Mahmoud
El-Badry
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt
Gihan
Fetih
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt
Ayat
Allam
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt
Mahmoud
Elsabahy
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt
Department of Chemistry, Laboratory for Synthetic-Biologic Interactions,Texas A&M University, College Station, TX, USA
Journal Article
2019
10
28
For transdermal delivery of meloxicam, nanostructured lipid carriers containing compritol as solid lipid, oleic acid as liquid lipid and different ratios of Pluronic F-68 were prepared. The prepared nanoparticles were characterized in terms of size, polydispersity index, zeta-potential and encapsulation efficiency. The average particle size, zeta-potential and encapsulation efficiency ranged from 134 to 491 nm, from -12.4 to -23.23 mV and from 35 to 70%, respectively. Furthermore, in vitro release for a number of selected formulations was performed using dialysis membrane in phosphate buffer saline. Drug release from free solution compared to release from nanostructured lipid carriers over a period of 48 hours was evaluated as well as release kinetic analysis was investigated. Moreover, stability of the selected formulation was studied at different time intervals. In addition, meloxicam-loaded nanostructured lipid carriers gel containing Carbopol-934 as a gelling agent was prepared. Moreover, anti-inflammatory activity of the prepared gel was evaluated using carrageenan-induced rat paw edema method. Meloxicamloaded nanostructured lipid carriers gel showed a more sustained inhibitory effect compared to free meloxicam gel. Finally, toxicity of the prepared meloxicam-loaded nanostructured lipid carriers gel was evaluated using histopathological examination. <br />
https://bpsa.journals.ekb.eg/article_62265_0be80946a85074c129e743d4abac82d7.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
42
1
2019
12
31
ENCAPSULATION OF ANTENNAPEDIA (PENETRATIN) PEPTIDE IN A POLYMERIC PLATFORM FOR EFFECTIVE TREATMENT OF INTRACELLULAR BACTERIA
63
70
62266
10.21608/bfsa.2019.62266
EN
Marwa
G. Elnaggar
Department of Industrial and Physical Pharmacy, Purdue University, 575 Stadium Mall Drive, West Lafayette, IN 47907, USA /Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
Hesham
M. Tawfeek
Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
Aly A.
Abdel-Rahman
Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
E. Aboutaleb
Ahmed
Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
Yoon
Yeo
Department of Industrial and Physical Pharmacy, Purdue University, 575 Stadium Mall Drive, West Lafayette, IN 47907, USA /
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
Journal Article
2019
11
04
Antimicrobial peptides (AMP) and cell-penetrating peptides (CPP) are two classes of peptides that share some structural and physicochemical similarities. Antennapedia or penetratin (ANT) is one of the most known CPPs, that was proven to have antimicrobial activity against certain strains of planktonic bacteria. ANT can enter the cells but has no activity against intracellular bacteria. This is attributable to the inability of the peptide to reach bacteria reside within cellular components as well as low delivery efficiency, due to loss of activity by proteolysis and poor specificity. The aim of this work is to develop a formulation that can effectively reach and attack intracellular bacteria. To achieve this goal, ANT was encapsulated in PLGA platform as nanoparticles with the size range of 500-1000 nm, which allows for selective uptake by macrophages where bacteria mostly reside. ANT was loaded with high loading efficiency (12.7%) inspite of high water solubility. ANT-nanoparticles (ANT-NP) had no cytotoxicity on J774a.1 macrophages and were readily taken up by macrophages as confirmed by fluorescence microscopy. Antibacterial activity of ANT-NP remains to be tested against different intracellular bacteria. <br /> <br />
https://bpsa.journals.ekb.eg/article_62266_0f330f6e2a5da740637b6629f17c1b20.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
42
1
2019
12
31
PERFORMANCE OF QUERCETIN-CHITOSAN GELS AS TOPICAL DELIVERY SYSTEMS
71
79
62273
10.21608/bfsa.2019.62273
EN
Nermin
ELeraky
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University 71526, Assiut, Egypt
Gihan
Fetih
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University 71526, Assiut, Egypt
Assiut International Center of Nanomedicine, Al-Rajhy Liver Hospital, Assiut University, Assiut 71515, Egypt
Mahmoud
El-Badry
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University 71526, Assiut, Egypt / Assiut International Center of Nanomedicine, Al-Rajhy Liver Hospital, Assiut University, Assiut 71515, Egypt
Journal Article
2019
11
05
This study was designed to evaluate suitability of chitosan polymer as a vehicle for topical delivery system. Quercetin (QUT) is a natural flavonoid, was incorporated into the gel vehicles in a concentration of 0.5% w/v. Gels were prepared with three different concentrations and different molecular weights of chitosan. Viscosity, drug release from prepared gels, permeation of drug through skin rat and anti-inflammatory activity of the drug using carrageenan induced rat paw edema method were studied. In-vitro release characteristics of the drug from different gels were carried out using dialysis membrane in phosphate buffer, pH 5.5. The release data were treated with various kinetic principles to assess the relevant parameters. The general rank order of QUT release was F1 > F4 > F2 > F3 > F5. The results also showed that, the release of drug from the prepared gels obeyed the diffusion model (Higuchi’s equation). The results revealed an inverse correlation between the drug release percent and the polymer concentration used. The permeation of drug through skin rat was carried out. The flux of drug is independent on the viscosity of the formulae. The results also showed a significant anti-inflammatory activity on rat paw edema
https://bpsa.journals.ekb.eg/article_62273_6b3b6c3005aae404e9227b42982cc6f7.pdf