Paper chromatographic separation of thiamine hydrochloride from its degradation products has been successfully carried out on Whatmann No. 1 chromatographic paper using n-butanol, acetic acid, acetone, water (45:20:10:25) as the developing system. For quantitative determination, the separated vitamin was eluted with acidic potassium chloride solution and determined spectrophotometrically at 250 nm. The recovery of thiamine hydrochloride was found to be satisfactory, (S.D. = 0.1017 & C.V. = 1.0184). The method was successfully applied for the determination of thiamine hydrochloride in synthetic mixtures and in single and multicomponent pharmaceutical products with good precision and almost complete recoveries.
Sina, A., Ibrahim, S., El-Sourady, H., & Shawky, S. (1978). A PAPER CHROMATOGRAPHIC METHOD FOR STABILITY INDICATING ASSAY OF THIAMINE HYDROCHLORIDE IN PHARMACEUTICAL PREPARATIONS. Bulletin of Pharmaceutical Sciences Assiut University, 1(1), 53-72. doi: 10.21608/bfsa.1978.104907
MLA
A. Sina; S. A. Ibrahim; H. A. El-Sourady; S. Shawky. "A PAPER CHROMATOGRAPHIC METHOD FOR STABILITY INDICATING ASSAY OF THIAMINE HYDROCHLORIDE IN PHARMACEUTICAL PREPARATIONS". Bulletin of Pharmaceutical Sciences Assiut University, 1, 1, 1978, 53-72. doi: 10.21608/bfsa.1978.104907
HARVARD
Sina, A., Ibrahim, S., El-Sourady, H., Shawky, S. (1978). 'A PAPER CHROMATOGRAPHIC METHOD FOR STABILITY INDICATING ASSAY OF THIAMINE HYDROCHLORIDE IN PHARMACEUTICAL PREPARATIONS', Bulletin of Pharmaceutical Sciences Assiut University, 1(1), pp. 53-72. doi: 10.21608/bfsa.1978.104907
VANCOUVER
Sina, A., Ibrahim, S., El-Sourady, H., Shawky, S. A PAPER CHROMATOGRAPHIC METHOD FOR STABILITY INDICATING ASSAY OF THIAMINE HYDROCHLORIDE IN PHARMACEUTICAL PREPARATIONS. Bulletin of Pharmaceutical Sciences Assiut University, 1978; 1(1): 53-72. doi: 10.21608/bfsa.1978.104907
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