PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND IN-VITRO ANTIDIABETIC ACTIVITY OF AN AYURVEDIC FORMULATION

Gayatri, S.; Monica, V. Angel Seslin

doi:10.21608/bfsa.2020.127400

PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND IN-VITRO ANTIDIABETIC ACTIVITY OF AN AYURVEDIC FORMULATION

Document Type : Original Article

Authors

S. Gayatri ¹
V. Angel Seslin Monica ²

¹ Assistant Professor, Sri Ramachandra Faculty of Pharmacy, SRIHER (DU), Porur, Chennai-600116, Tamil Nadu, India

² Student, Sri Ramachandra Faculty of Pharmacy, SRIHER (DU), Porur, Chennai-600116, Tamil Nadu, India

10.21608/bfsa.2020.127400

Abstract

Objective: To carry out the preliminary phytochemical analysis and to perform the in-vitro anti diabetic activity for the ayurvedic poly herbal formulation.
Material and Methods: Preliminary phytochemical analysis was performed as per the standard procedures. In-vitro anti-diabetic activity was done by two methods namely alpha amylase inhibitory assay and alpha glucosidase inhibitory assay by taking acarbose as a standard drug for the ayurvedic formulation. Statistical analysis is carried out by means of graphpad prism software version 6.
Results and conclusion: Preliminary phytochemical analysis showed the presence of alkaloids, glycosides, phytosterols, phenols, flavonoids and terpenoids in the formulation. The poly herbal tablet produced 32.2% inhibition of alpha amylase activity at a concentration of 125 µg/ml and 80.8% inhibition of alpha glucosidase activity at a concentration of 62.5 µg/ml. The present study shows that the selected ayurvedic poly herbal tablets has an anti-diabetic activity comparable with that of allopathic drug - acarbose tablet.