CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF ALLIUM SATIVUM L. ESSENTIAL OIL AGAINST MDR BACTERIA ISOLATED FROM BUCCAL CAVITY AFFECTED BY CARIES

Imane, Ould Amer; Selles, Sidi Mohammed Ammar; Hocine, Laaredj; Abdelkader, Dilmi Bouras; Yamina, Kadari

doi:10.21608/bfsa.2023.327628

CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF ALLIUM SATIVUM L. ESSENTIAL OIL AGAINST MDR BACTERIA ISOLATED FROM BUCCAL CAVITY AFFECTED BY CARIES

Document Type : Original Article

Authors

¹ Faculty of Life and Nature Sciences, University of Tiaret, Tiaret 14000, Algeria Laboratory of Agro-Biotechnology and Nutrition in Semi-Arid Areas, University of Tiaret, Tiaret 14000, Algeria

² Institute of veterinary sciences, University of Tiaret, Tiaret 14000, Algeria

³ Faculty of Life and Nature Sciences, University of Tiaret, Tiaret 14000, Algeria

⁴ Labotatory of natural bioressources, Université of Chlef Hassiba Benbouali, Chlef 2000, Algeria

⁵ Labotatory of natural bioressources, Université of Chlef Hassiba Benbouali, Chlef 2000, Algeria Laboratory of Farm Animal Reproduction, University of Tiaret, Algeria

10.21608/bfsa.2023.327628

Abstract

Natural remedies have proven to be very effective and have fewer side effects than commercial antibiotics. The objectives of this work were to determine the chemical compounds and biological (antibacterial and antioxidant) activities of Allium sativum L. essential oil against three multi-drug strains of bacteria isolated from buccal cavity affected by caries namely Aerococcus viridans, Staphylococcus epidermidis, and S. xylosus. The essential oil was extracted by hydrodistillation, chemical compounds were quantified by gas chromatography-mass spectrometry and gas chromatography with flame ionization detection. 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing power were used to study antioxidant activity. Antibacterial activity was carried out by the disk diffusion method. The average yield of the essential oil was 0.173±0.009% (w/w). All tested strains were resistant to three different classes of antibiotics. Diallyl disulfide (39.22%) and diallyl trisulfide (34.85%) were the main components of Allium sativum L. essential oils. DPPH radical scavenging assay showed the median inhibitory concentration (IC₅₀) value of 51.12±11.77 mg/ml, while the ferric reducing power assay recorded the median effective concentration (EC₅₀)value of 6.54 ± 0.63 mg/ml. The results showed that all multidrug-resistant bacteria strains tested are sensitive to essential oils. The results indicate that Allium sativum L. essential oil exercises good in-vitro antibacterial and weak to moderate in-vitro antioxidant activities.

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