SPECTROPHOTOMETRIC DETERMINATION OF PIPERAZINE USING 3,5-DIBROMO-2-METHYL-p-BENZOQUINONE

Mohamed, H. A.; Khashaba, P. Y.; Mohamed, A. I.; El-Shorbagi, A. A.

doi:10.21608/bfsa.1993.70041

SPECTROPHOTOMETRIC DETERMINATION OF PIPERAZINE USING 3,5-DIBROMO-2-METHYL-p-BENZOQUINONE

Document Type : Original Article

Authors

¹ Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt

² Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt

10.21608/bfsa.1993.70041

Abstract

A spectrophotometric method is developed for the quantitative determination of piperazine either in bulk drug or in pharmaceutical dosage forms. The method is based on the formation of vinylamine of this drug by interaction with acetaldehyde. The resulting enamine interacts with 3,5-dibromo, 2-methyl-p-benzoquinone (DEMQ) forming an intense blue colored aminovinylquinone which can be estimated spectrophotometrically at l_m_a_x642 nm. DEMQ is prepared according to a modified oxidation procedure by treatment of 2,4,6 tribromo-3-methylphenol with chromic oxide in a mixture of acetic acid and acetonitrile (9:1). Beer-Lambert's Law was adhered to over the range of 2-8, 2-9, and 2-10 mg.ml^-1 for piperazine hydrate, tartrate, citrate and phosphate respectively. The optimum reaction conditions and the effect of all variables have been studied. The proposed method is simple, rapid, sensitive, accurate, precise and has been applied successfully for the analysis of some pharmaceutical formulations of piperazine including tablets, effervescent granules and syrup with good recoveries. Results obtained are in good agreement with those obtained by the official methods.