@article { author = {Soliman, Isamil and Ahmed, Ahmed and Deaf, Enas and Sayed, Ibrahim}, title = {NOSOCOMIAL ACINETOBACTER INFECTION IN ASSIUT UNIVERSITY HOSPITAL}, journal = {Bulletin of Pharmaceutical Sciences Assiut University}, volume = {35}, number = {2}, pages = {99-107}, year = {2012}, publisher = {Assiut University, Faculty of Pharmacy}, issn = {1110-0052}, eissn = {3009-7703}, doi = {10.21608/bfsa.2012.64608}, abstract = {Aim:This study aimed to isolation of Acinetobacter spp, detect resistance patterns of isolated strains, phenotypic and genotypic detection of Metallo-β-lactamase and typing of isolated strains from clinical and environmental respectively. Material and method: this study includes 440 different clinical specimens, 672 environmental samples, inoculated on different culture media, confirmation of isolates by API20NE, PCR to detect 16SrRNA-23SrRNA gene, determine susceptibility pattern of isolates to different antibiotics and phenotypic and Genotypic detection of bla –OXA 51-like gene. Result: 24 strains of Acinetobacter (5.45%) were isolated from 440 clinical samples, 27 strains of Acinetobacter were isolated from 672 environmental samples (4.017%). Tetracycline was the most active drug against multi-drug resistant A.baumannii. (48/51 or 94%) of Acinetobacter isolates showed increase in zone of inhibition around IPM/EDTA disc compared with IPM disc alone. (49/51 or 96%) of Acinetobacter isolates were detected by presence of 16srRNA 23srRNA gene (universal gene present in all Acinetobacter species). (49/51 or 96%) of isolated Acinetobacter spp showed band with blaOXA-51-like”genes. (37/51 or 72.5%) Acinetobacter isolates showed positive bands for class I integrase gene (gene responsible for multi drug resistance and outbreaks in hospitals).}, keywords = {}, url = {https://bpsa.journals.ekb.eg/article_64608.html}, eprint = {https://bpsa.journals.ekb.eg/article_64608_44bf388ec72ba2eb0813d78e8cf685f3.pdf} } @article { author = {Ali, A. and Sayed, H. and Mohamed, S. and Zaher, A.}, title = {MACRO- AND MICROMORPHOLOGY OF THE ROOT, STEM, LEAF, INFLORESCENCE AND FRUIT OF EUPHORBIA PEPLUS L. GROWING IN EGYPT}, journal = {Bulletin of Pharmaceutical Sciences Assiut University}, volume = {35}, number = {2}, pages = {109-126}, year = {2012}, publisher = {Assiut University, Faculty of Pharmacy}, issn = {1110-0052}, eissn = {3009-7703}, doi = {10.21608/bfsa.2012.64610}, abstract = {Euphorbia peplus L. belongs to Family Euphorbiaceae which includes about 283 genera with almost 7500 species. They are distributed all over the world mainly tropical countries. Some species of the genus Euphorbia showed antiviral and anticancer activities. It was reported to be used in folkloric medicine as purgative and in treatment of skin diseases, gonorrhea, liver disorders, chest diseases, and gout. Some phytochemical studies have been carried out abroad on different species. The authors carried out phytochemical and biological studies on the studied plant and here in we undertake macro- and micromorphological studies with the aim of finding out the diagnostic features by which the plant could be identified in both entire and powdered forms.}, keywords = {}, url = {https://bpsa.journals.ekb.eg/article_64610.html}, eprint = {https://bpsa.journals.ekb.eg/article_64610_49b5f4c3c9a81f889d709a65da3e41f9.pdf} } @article { author = {Makboul, M. and Attia, A. and Farag, S. and Kamal, N.}, title = {MACRO- AND MICROMORPHOLOGY OF THE AERIAL PARTS OF LANTANA MONTEVIDENSIS (SPRENG.) BRIQ. CULTIVATED IN EGYPT}, journal = {Bulletin of Pharmaceutical Sciences Assiut University}, volume = {35}, number = {2}, pages = {127-160}, year = {2012}, publisher = {Assiut University, Faculty of Pharmacy}, issn = {1110-0052}, eissn = {3009-7703}, doi = {10.21608/bfsa.2012.64732}, abstract = {Lantana montevidensis (Spreng.) Briq. is a member of family Verbenaceae, that includes about 98 genera and more than 2600 species which are predominantly tropical or subtropical plants. The leaf infusions of the plant are used in folk medicine to treat fever, influenza, asthma, bronchitis, and many other diseases. Biological studies showed that the plant has antimicrobial and antiproliferative activities. No detailed information could be traced concerning the macroand micromorphology of the plant. This motivated the authors to carry out this study to identify the drug in both entire and powdered form.}, keywords = {}, url = {https://bpsa.journals.ekb.eg/article_64732.html}, eprint = {https://bpsa.journals.ekb.eg/article_64732_adaef743df3b5f3392e283b921134c7b.pdf} } @article { author = {El-Sayed, Ahmed and Abd El-Mohsen, Mohamed and Mohamed, Mohamed and Rafat, Nafisa and Sayed, Ola}, title = {COMPATIBILITY AND STABILITY OF TERNARY ADMIXTURE OF MIDAZOLAM, DOBUTAMINE AND DOPAMINE IN 5% GLUCOSE OR 0.9% SODIUM CHLORIDE SOLUTION}, journal = {Bulletin of Pharmaceutical Sciences Assiut University}, volume = {35}, number = {2}, pages = {161-180}, year = {2012}, publisher = {Assiut University, Faculty of Pharmacy}, issn = {1110-0052}, eissn = {3009-7703}, doi = {10.21608/bfsa.2012.64877}, abstract = {The aim of this study was to evaluate compatibility and stability of the maximum concentration used for ternary admixture containing midazolam, dobutamine and dopamine in 5% glucose and 0.9% sodium chloride solutions. The maximum concentration of each drug was 0.144 mg/ml of midazolam, 5.76 mg/ml of dobutamine and 2.88 mg/ml of dopamine in 50 ml of 5% glucose or 0.9% sodium chloride solutions. The physical compatibility of ternary admixtures was assessed using visual inspection and pH determination of ternary admixtures immediately after preparation (at 0 time) and after 24 hrs. The chemical stability was assessed using high performance thin layer chromatoghraphy (HPTLC). The method is based on HPTLC separation of the three drugs followed by densitometric measurements of their spots at 254 nm using Camag TLC Scanner 3. The mobile phase comprised ethyl acetate : n-propanol : water : glecial acetic acid (60:24:9:3, v/v/v/v). There were no visual changes (such as precipitation, gas evaluation or change in color) during 24 hrs after preparation of admixture. Also, there was no change in pH values of admixtures during that time. The results revealed chemical stability of midazolam, dobutamine and dopamine over the duration of mixing (24 hrs) in 5% glucose or 0.9% sodium chloride solutions}, keywords = {}, url = {https://bpsa.journals.ekb.eg/article_64877.html}, eprint = {https://bpsa.journals.ekb.eg/article_64877_7e281c11a20b8d8e5be01c082a2c1022.pdf} } @article { author = {Ismail, S. and Mohammed, F. and Ismail, M.}, title = {FORMULATION AND EVALUATION OF SOME SELECTED TIMOLOL MALEATE OPHTHALMIC PREPARATIONS}, journal = {Bulletin of Pharmaceutical Sciences Assiut University}, volume = {35}, number = {2}, pages = {181-197}, year = {2012}, publisher = {Assiut University, Faculty of Pharmacy}, issn = {1110-0052}, eissn = {3009-7703}, doi = {10.21608/bfsa.2012.64878}, abstract = {The purpose of this study was to prepare and evaluate certain Timolol maleate (TM) polymeric formulations including viscous solutions, hydrogels and in-situ gels aiming to improve its ocular bioavailability and decrease its side effects. In this study, Chitosan (CS), hydroxyehtylcellulose (HEC), hydroxypropylmethylcellulose (HPMC) and sodium carboxymethylcellulose (NaCMC) were used to prepare TM viscous solutions and hydrogels. In-situ gels of Gelrite and Pluronic F-127 (Pl F-127) were prepared at different concentrations. Mucoadhesives namely CS or HEC were incorporated to Pl F-127 to produce mucoadhesive/insitu gel. The prepared formulations were evaluated for their in-vitro drug release, viscosity, gelation temperature and mucoadhesive force. The selected TM formulations were tested for their effect on intraocular pressure (IOP) and systemic side effects based on blood pressure (BP), heart rate (HR) and respiration rate (RR). The results revealed that, TM in situ-gels F25 (containing Pl F-127 20% and CS 1.5% w/w) and F31(containing Gelrite 0.6% w/w) showed reasonable in-vitro results, and a marked IOP lowering activity without systemic side effects compared to TM marketed eye drops.}, keywords = {}, url = {https://bpsa.journals.ekb.eg/article_64878.html}, eprint = {https://bpsa.journals.ekb.eg/article_64878_4badd84a9004b5cafdc3b4875750cbdf.pdf} } @article { author = {Saleh, Gamal and Badr, Ibrahim and Derayea, Sayed and Nour El-Deen, Deena}, title = {BATCH AND FLOW INJECTION POTENTIOMETRIC MONITORING OF CEFTRIAXONE SODIUM IN PHARMACEUTICAL PREPARATIONS USING TWO NOVEL MEMBRANE SENSORS}, journal = {Bulletin of Pharmaceutical Sciences Assiut University}, volume = {35}, number = {2}, pages = {199-214}, year = {2012}, publisher = {Assiut University, Faculty of Pharmacy}, issn = {1110-0052}, eissn = {3009-7703}, doi = {10.21608/bfsa.2012.64879}, abstract = {Two novel potentiometric sensors are prepared, characterized and successfully used for static and continuous determination of ceftriaxone sodium (CRXN). Both sensors are based on the use of plasticized PVC matrix membranes incorporating tetradodecylmethyl ammonium bromide (TDMAB), ortridodecylmethyl ammonium chloride (TDMAC) ion-exchangers and used for quantitative determination of CRXN at concentration level down to 29 µM using both sensors with a good accuracy. Both sensors offer the advantages of fast response, reasonable selectivity, elimination of drug pre-treatment or separation steps, low cost and possible interfacings with computerized and automated systems. The use of plasticized membrane electrodes were used for continuous monitoring of CRXN offers the advantages of simple design, ease of construction and possible applications to small volumes of drug solutions with little manipulation and without pre-treatment. Both detectors display a wide dynamic measurement range of the drug under continuous mode of operation with a flow rate of 2.0 ml.min-1 and used for quantitative determination of CRXN. The developed sensors were utilized in static continuous modes successfully for the determination of CRXN in pure powders and in dosage forms. It is worth noting that the developed membrane electrodes exhibited good selectivity toward CRXN over other cephalosporins such as; cefradine, ceftazidime, cefadroxil, cefaclor and cefoperazone, as well as other additives found in the pharmaceutical preparations such as; glucose, fructose and maltose.}, keywords = {}, url = {https://bpsa.journals.ekb.eg/article_64879.html}, eprint = {https://bpsa.journals.ekb.eg/article_64879_b8f16e9a27bbf70db979ec4bb0917f67.pdf} }