ORIGINAL_ARTICLE
PREVALENCE OF NOSOCOMIAL INFECTIONS CAUSED BY PSEUDOMONAS AERGINOSA IN ASSIUT UNIVERSITY HOSPITAL
Pseudomonas (Ps.) aeruginosa is one of the most common pathogens causing nosocomial infections. This pathogen causes several infections including urinary tract infection (UTI), wound infection, pneumonia, bacteremia,…etc. Immunocompromised patients and patients in intensive care unit are at high risk of acquisition of infection, in addition multidrug resistant Ps. aeruginosa isolates had been characterized. This study was planned to determine the frequency of Ps. aeruginosa in nosocomially infected patients in Assiut university hospital and to type isolated strains. In this study, 677 samples collected from 366 nosocomially infected patients admitted to different hospital wards at Assiut University Hospitals, including chest, trauma, neurology, internal medicine, post operative and pediatric ICUs, trauma and hematology units. Identification of bacterial strains was done by cultural and biochemical tests. Biotyping analysis for isolated strains was done using API 20NE. In our study, a total of 30 (8.2%) Ps. aeruginosa strains were isolated. Four API codes profile for Ps. aeruginosa isolates were identified, the isolate with API code profile 1154575 was more frequent distributed in Assiut University Hospital.
https://bpsa.journals.ekb.eg/article_63160_72be61df9128abcd8fe8c1db87b44540.pdf
2017-12-01
1
8
10.21608/bfsa.2017.63160
Ismail
Soliman
1
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
LEAD_AUTHOR
Ehsan
Hassan
2
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
LEAD_AUTHOR
Noha
Afifi
3
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
LEAD_AUTHOR
Sherin
Abd El-Rahman
4
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
LEAD_AUTHOR
Ayat
Kamel
5
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
LEAD_AUTHOR
ORIGINAL_ARTICLE
MACRO- AND MICROMORPHOLOGY OF THE LEAVES, STEMS, SEEDS AND FRUITS OF IPOMOEA ERIOCARPA (R. Br.) GROWING IN EGYPT
Ipomoea eriocarpa R.Br. belongs to family Convolvulaceae often called morning glories, are summer annual or perennial broadleaf plants. They are slender twining herb. It is occasionally consumed as an edible leafy vegetable or mixed with other food. An oil extract of Ipomoea eriocarpa is used for external application in headache, rheumatism, leprosy, ulcers and fevers. By reviewing the current literature, no botanical studies were done on Ipomoea eriocarpa R.Br., so this study aims to characterization and identification of this plant in both powdered and entire forms.
https://bpsa.journals.ekb.eg/article_63162_32e23f0705ad6b6f26589aa0d8743de3.pdf
2017-12-31
9
31
10.21608/bfsa.2017.63162
A.
Khalifa
1
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
LEAD_AUTHOR
A.
Mohamed
2
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
LEAD_AUTHOR
Z.
Ibrheim
3
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
LEAD_AUTHOR
A.
Hamoda
4
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
LEAD_AUTHOR
ORIGINAL_ARTICLE
FORMULATION AND EVALUATION OF BUCCOADHESIVE SUSTAINED-RELEASE DISCS OF GLIPIZIDE
Glipizide is an oral hypoglycemic agent used in the treatment of type II diabetes mellitus. It is characterized by its poor aqueous solubility and delayed absorption with concomitant food intake. The objective of the present study was to enhance the absorption rate of glipizide and avoid its side effects on stomach by formulating it into buccoadhesive sustained release disc formulations. The discs were prepared by direct compression method. Hydroxypropyl methylcellulose (HPMC 15000), was used as the main hydrophilic matrix forming polymer either alone or in combinations in two ratios (3:2 & 4:1) with various mucoadhesive polymers namely; Sodium alginate (NaAlg), Sodium carboxymethyl cellulose (SCMC), Hydroxyethyl cellulose (HEC), Hydroxypropyl cellulose (HPC) and Chitosan. The discs were evaluated for weight variation test, thickness, diameter, drug content, hardness, friability, swelling index, surface pH, in-vitro bioadhesion, in-vitro release studies and in-vivo bioavailability studies. Invitro release studies demonstrated that formulation F8 which contains HPMC / SCMC (40%: 10%) has sustained the drug release up to 8 hrs which was considered an optimum pattern of drug release. The kinetic studies revealed that all formulations follows zero order release kinetics except F3, F4 and F11 which fitted well in first order release model. Bioavailability parameters including Cmax, Tmax, and AUC0–24 h of F8 and the commercial oral tablets of glipizide (Minidiab® 5 mg) were compared. The selected formulation F8 produced higher Cmax and extended Tmax (P<0.05).
https://bpsa.journals.ekb.eg/article_63163_552d7e0090a110d0bc276ea6cc03b424.pdf
2017-12-31
33
47
10.21608/bfsa.2017.63163
Ahmed
Aboutaleb
1
Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
LEAD_AUTHOR
Aly
Abdel-Rahman
2
Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
LEAD_AUTHOR
Sayed
Khedr
3
Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
LEAD_AUTHOR
Gamal
Elattar
4
Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
LEAD_AUTHOR
ORIGINAL_ARTICLE
PREVALENCE OF KLEBSIELLA PNEUMONIAE CAUSING VENTILATOR-ASSOCIATED PNEUMONIA (VAP) ISOLATED FROM PEDIATRIC INTENSIVE CARE UNIT AT ASSIUT UNIVERSITY HOSPITAL
Ventilator-associated pneumonia is a common nosocomial infection occurring in patients receiving mechanical ventilation especially in pediatric intensive care units (PICUs). Klebsiella pneumoniae is a multidrug resistant nosocomial pathogen that plays an important role in respiratory tract infection among critically ill patients. This study included 51 pediatric patients had VAP admitted to the Pediatric Intensive Care Unit (PICU) at Assiut university children's hospital through 12-month period from May 2014 to May 2015, using quantitative endotracheal aspirate (EA) culture. K. pneumoniae were isolated by culturing onto blood and MacConkey`s agar plates then incubated aerobically at 35°C for 2448 hrs. and identified by morphology and biochemical tests. K. pneumoniae 19 (37.25%) was the most isolated bacteria that causing VAP in PICU. Antimicrobial susceptibility testing showed that K. pneumoniae isolates were resistant to all commercial available antimicrobial agents that used in the PICU.
https://bpsa.journals.ekb.eg/article_63165_0c21700640d40d4247844ce55d6ae334.pdf
2017-12-31
49
56
10.21608/bfsa.2017.63165
Eman
Mohamed
1
Department of Microbiology and Immunology, Faculty of pharmacy, Al –Azhar University, Assiut branch
LEAD_AUTHOR
Hamada
Mohamed
2
Department of Microbiology and Immunology, Faculty of pharmacy, Al –Azhar University, Assiut branch
AUTHOR
Sherine
Abd El- Rahman
3
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
AUTHOR
Shabaan
Hashem
4
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
AUTHOR
ORIGINAL_ARTICLE
FORMULATION AND EVALUATION OF ONDANSETRON TRANSDERMAL GELS
The aim of this study was to develop and evaluate ondansetron gels for transdermal effect using in-vitro and ex-vivo permeation methods. Ondansetron gels were prepared using; sodium carboxymethyl cellulose (Na CMC), hydroxypropylmethyl cellulose (HPMC), hydroxypropyl cellulose (HPC), sodium alginate, and pluronic F- 127 as gelling polymers. Polyvinyl pyrrolidone (PVP) and polyethylene glycol (PEG) 400 were used as solubility enhancers. Oleic acid, ethyl alcohol, menthol, isopropyl myristate and propylene glycol were evaluated as penetration enhancers. The effect of the employed gel bases and permeation enhancers on the physicochemical characterization and drug permeation through cellophane membrane and rat skin were determined. The results showed that both polymers and their concentrations affect the permeation of drug, by increasing the polymer concentration in the formulation, viscosity increased and invitro permeation of ondansetron decreased. Formula containing 16% of hydroxyl propyl cellulose with PVP/PEG400 (1.5/5% w/w) showed the highest amount permeation (95% over a 6 hrs period) through cellophane membrane. Both formulae containing 16% of hydroxyl propyl cellulose with PVP/PEG400 (1.5/5% w/w) and 7% of sodium alginate with PVP/ PEG400 (1.5/5% w/w) showed the best permeation through rat skin (amount permeated about 345.06 µg/cm2) over a 6-hr period. PVP/PEG400 (1.5/5% w/w) showed the optimum solubilization enhancement.
https://bpsa.journals.ekb.eg/article_63166_8c57983eca6131ffb21228dda095cdc9.pdf
2017-12-31
57
70
10.21608/bfsa.2017.63166
Mai
Rasheedy
1
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
LEAD_AUTHOR
Mona
El-Mahdy
2
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
AUTHOR
Dina
Fathallah
3
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
AUTHOR
Elsayed
Ibrahim
4
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
AUTHOR
ORIGINAL_ARTICLE
BOTANICAL AND BIOLOGICAL STUDY OF THE LEAVES OF CARYOTA MITIS LOUR. FAMILY ARECACEAE CULTIVATED IN EGYPT
The macromorphological description of stem, leaves, inflorescences, flowers and fruits in addition to micromorphological study of leaves and primary axis of Caryota mitis lour. Family Arecaceae cultivated in Egypt were carried out for the identification of the plant in both entire and powdered forms. Evaluation of the LD50 and pharmacological activities of the dried powdered leaves extracts revealed analgesic, antipyretic in addition to antiinflamatory activities.
https://bpsa.journals.ekb.eg/article_63167_6e3f74b12d67c2515d699771c5f4f589.pdf
2017-12-31
71
95
10.21608/bfsa.2017.63167
Islam Abd Elhakim
Abd Elhakim
1
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
LEAD_AUTHOR
Afaf
Abdel-Baky
2
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
D.
Bishay
3
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR