ORIGINAL_ARTICLE
A NEW SECOIRIDOID GLUCOSIDE FROM JASMINUM AZORICUM L.
A new secoiridoid glucoside has been isolated from the methanolic fraction of the leavesof Jasminum azoricum L. The structure of the isolated compound have been identified byspectral analysis.
https://bpsa.journals.ekb.eg/article_65462_1c3bb159e3b90c135822654a9c730685.pdf
2003-06-30
1
3
10.21608/bfsa.2003.65462
A.
Attia
1
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
A.
Abd El-Mawla
2
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
ORIGINAL_ARTICLE
MULTIVARIATE SPECTROPHOTOMETRIC ANALYSIS OF CERTAIN ANTIHYPERTENSIVE BINARY MIXTURES
A multivariate calibration method for the analysis of two hypotensive binary mixtures isdescribed. The mixtures are composed of reserpine in combination either withhydrochlorothiazide or hydrofluomethiazide respectively. The components of the two mixturesshow a considerable degree of spectral overlapping (83.5-88.5%). Resolution of the binarymixtures under investigation has been accomplished mainly by using classical least squares(CLS) analysis. The mixtures are simultaneously determined in three laboratory prepared tabletforms with high accuracy without interference from the commonly added excipients. Goodrecoveries were obtained with both synthetic mixtures and prepared tablets. The obtainedresults were compared with those obtained from official methods and found in good agreementwith them. A comparison of the obtained results from CLS were performed with those obtainedfrom principle component regression (PCR).
https://bpsa.journals.ekb.eg/article_65463_561330a142a7cee853b347d71ac5b1e6.pdf
2003-06-30
5
13
10.21608/bfsa.2003.65463
Abd El-Maaboud
Mohamed
1
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt
LEAD_AUTHOR
Samiha
Hussein
2
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
Hanaa
Abdel-Wadood
3
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
ORIGINAL_ARTICLE
THE USE OF QUERCETIN FOR SPECTROPHOTOMETRIC DETERMINATION OF SOME CNS ACTING DRUGS
Two simple and sensitive spectrophotometric methods for the determination of seven CNSacting drugs namely; haloperidol, droperidol, chlorpromazine hydrochloride, thioridazinehydrochloride, imipramine hydrochloride, desipramine hydrochloride and clomipraminehydrochloride are presented. The first method is based on formation of an ion-pair complexbetween each of studied drugs and oxidized quercetin at pH range from 4.5 to 5.0. The coloredcomplex formed was quantitatively extracted into dichloroethane, filtered over anhydroussodium sulphate and then measured in the range of 528-534 nm. Beer's law was obeyed for allthe studied drugs in the concentration range of 2-45 μg/ml. The second method is a rapidkinetic method based on in-situ oxidation of quercetin into a red colored compound measuredat 515 nm. The rate of reaction between each of studied drugs and oxidized quercetin wasfollowed by measuring the decrease in absorption intensity as a result of its reduction by eachof the studied drugs. The studied compounds were determined in the concentration range of 5-50 μg/ml using slope, fixed time and variable time methods. The two methods were successfullyapplied to the analysis of studied drugs in the pure form and in commercial pharmaceuticals.The results were comparable with those obtained with either official or reported methods.
https://bpsa.journals.ekb.eg/article_65464_84c58f3e0bb541f8f5df4cbd5b99d705.pdf
2003-06-30
15
27
10.21608/bfsa.2003.65464
F.
Mohamed
1
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut-71526, Egypt
AUTHOR
S.
Hussein
2
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut-71526, Egypt
AUTHOR
H.
Mohamed
3
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut-71526, Egypt
AUTHOR
S.
Ahmed
4
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut-71526, Egypt
AUTHOR
ORIGINAL_ARTICLE
MUCOADHESIVE VAGINAL TABLETS OF NATAMYCIN FOR VAGINAL CANDIDIASIS
Bioadhesive vaginal tablets of natamycin were formulated with different bioadhesivepolymers such as carbopol 971p, carbopol 974p, hydroxypropyl methylcellulose, and sodiumcarboxymethylcellulose. Physical tests were applied to the tablets. The bioadhesive tablets wereevaluated for drug release rate and swelling behaviour in citrate buffer solution andbioadhesive strength to mucosal tissue of cow vagina. The swelling of the tablets containingcarbopol 974p was found to last for up to 6 hr in citrate buffer solution. The force necessary todetach the tablets from the vaginal tissue was found to be dependent on the concentration andtype of the bioadhesive polymer. The bioadhesive polymers were found to decrease the drugrelease rate. However, inclusion of polyethylene glycol 6000 in the formulations increased thereleased amount of the drug from 6.35% to 38.83% after 12 h. The bioadhesive tablets wereclinically evaluated in volunteers suffering from vaginal candidiasis and compared withconventional natamycin vaginal tablets. It was found that the bioadhesive tablets were moreeffective than the conventional tablets as they lasted about 4 days in the vaginal cavity and thiscould sustain the drug release and lead to better patient compliance. Accelerated stabilitystudies were carried out and showed that the selected formulations were stable for not less than2.5 years.
https://bpsa.journals.ekb.eg/article_65465_0ae3073aa732795c64b992ce43a4e9c7.pdf
2003-06-30
29
40
10.21608/bfsa.2003.65465
G.
El-Gindy
1
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
LEAD_AUTHOR
A.
El-Sayed
2
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
AUTHOR
S.
Mohame
3
Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University, Assiut, 71526, Egypt
AUTHOR
Gh.
Abdel-Aal
4
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
AUTHOR
ORIGINAL_ARTICLE
PHENOLIC AND ALIPHATIC GLUCOSIDES FROM CRYPTOSTEGIA GRANDIFLORA AND CARDIOTONIC ACTIVITY OF CRYPTOSTIGMIN II
From the leaves of Cryptostegia grandiflora, two phenolic glucosides 2,4,6-trihydroxybenzophenone 2-O- -glucopyranoside and Acanthoside B together with amegastigmane (Icariside B1) and (Z)-3-hexenyl -D-glucopyranoside have been isolated.Moreover the cardiotonic activities of Cryptostegia extract and Cryptostigmin II, the majorcardenolide previously isolated from the same plant leaves were also investigated. The lattershowed similar effects to those of Digoxin
https://bpsa.journals.ekb.eg/article_65466_67661718c6d663a38738c786068cc30b.pdf
2003-06-30
41
48
10.21608/bfsa.2003.65466
M.
Assaf
1
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
AUTHOR
M.
Kamel
2
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
AUTHOR
D.
Bishay
3
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
AUTHOR
ORIGINAL_ARTICLE
ANTHRAQUINONES PRODUCTION IN AERIAL PARTS-DERIVED CALLUS OF RUBIA TINCTORUM L.
Callus cultures were developed from the aerial parts of Rubia tinctorum L. on MS mediumcontaining 6-benzylaminopurine and 1-naphthaleneacetic acid. The changes in callus weightand anthraquinones content were followed between week 1 and 6. Anthraquinones content wasmonitored by HPLC. The linear increase in fresh weight was found to be parallel to theanthraquinones production. Effect of medium pH, incubation temperature and growthregulators on callus growth and anthraquinones content was studied and optimised. pH 7.5 andtemperature of 30° were found optimum for the callus growth. 6-Benzylaminopurine (0.5 mg/L)and 1-naphthaleneacetic acid (2.0 mg/L) were found the most appropriate growth regulators.The callus ethanolic extract showed the same picture on TLC as that of the roots. Theanthraquinones alizarin (1), lucidin--ethyl ether (2), 1-hydroxy-2-hydroxymethylantraquinone-3-O- -glucoside (3) and lucidin primeveroside (4) in addition to other four minoranthraquinone spots were found to be produced by these cultures. The four isolated compounds(1-4) were identified by spectral analysis (UV, IR and MS) as well as comparison with authenticsamples. Aerial parts, roots and the callus were analysed for their anthraquinones content. Theanthraquinones content of callus extract was about 91% and eighteen fold of the roots andaerial parts extract respectively.
https://bpsa.journals.ekb.eg/article_65467_5c99bd1a639eeed849c38bf49b1d9819.pdf
2003-06-30
49
54
10.21608/bfsa.2003.65467
A.
Abd El-Mawla
1
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
E.
Backheet
2
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
N.
El-Emary
3
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
ORIGINAL_ARTICLE
FLAVONOIDS AND CYANOGENIC GLYCOSIDES FROM THE LEAVES AND STEM BARK OF PRUNUS PERSICA (L.) BATSCH (MEET GHAMR) PEACH LOCAL CULTIVAR IN ASSIUT REGION
Mandelonitrile- -D-glucopyranoside (prunasin) (1), kaempferol- 3-O- -Dgalactopyranoside(2), kaempferol-3-O- -D-glucopyranoside (3), quercetin-3-O- -Dglucopyranoside(4), mandelic acid amide- -D-glucopyranoside (5), kaempferol-3-O-[ -Dglucopyranosyl-(14)--D-galactopyranoside] (6), mandelonitrile- -D-glucopyranosyl-(16)- -D- glucopyranoside (amygdalin) (7) were isolated from the methanolic extract of theleaves of Prunus persica (L.) Batsch “Meet Ghamr” peach. Furthermore, persicogenin (8),naringenin (9), aromadendrin (10), eriodictyol (11), persicogenin-3-O- -D-glucopyranoside(12) and hesperitin-5-O- -glucopyranoside (13) were isolated from the methanolic extract ofthe stem bark of the title plant. All these compounds were isolated for the first time from “MeetGhamr“ peach while compounds 5 and 6 were firstly reported from the genus Prunus.Identification of these compounds has been established by physical, chemical and spectralmethods (UV, IR, FAB-MS, 1-D-and 2-D NMR).
https://bpsa.journals.ekb.eg/article_65468_8e82850c94f5f358b43d5367ff8f7afa.pdf
2003-06-30
55
66
10.21608/bfsa.2003.65468
Enaam
Backheet
1
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
Salwa
Farag
2
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
Amany
Ahmed
3
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
Hanaa
Sayed
4
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
ORIGINAL_ARTICLE
ECBALLIUM ELATERIUM; A POSSIBLE TOPICAL ANTI-INFLAMMATORY DRUG
Owing to the toxicity of Ecballium elaterium (EB) when taken orally, the aim of this investigation was to prepare gel formulations of this drug for the treatment of inflammation. The anti-inflammatory activities of EB extract was studied in comparison with Diclofenac sodium market topical gel (Diclogesic gel®), using the carrageenan induced paw edema model in male albino rats. The pharmacological screening revealed that the percent reduction of edema produced by EB extract (85.32%) was lower than that of Diclogesic gel® (78.78%), i.e. it is more effective as anti-inflammatory agent than the market diclofenac sodium. Three types of gel formulations were prepared containing 1, 2 or 3% of EB in three gel bases, namely; Sodium carboxymethyl-cellulose (NaCMC), Plantago seed husks (PT), and Sodium alginate (NaAlg). EB 3% in NaCMC-gel was found to have comparatively the highest releasing and penetration power through the cellophane membrane and hairless mouse skin, respectively. NaCMC-gel showed similar diffusion coefficients (Dapp) to PT- gel (1-1.5), while NaAlg preparations showed lower results (0.5). However, the permeability coefficient results revealed that NaCMC formulations have higher values followed by PT and NaAlg. It could be concluded that EB is a promising topical anti-inflammatory agent.
https://bpsa.journals.ekb.eg/article_65469_51f7cc43e28bf3cdb70a67a7ee0e251f.pdf
2003-06-30
67
76
10.21608/bfsa.2003.65469
Adel
Aly
1
Alisra University, Amman, Jordan
AUTHOR
Mazen
Qato
2
Alisra University, Amman, Jordan
AUTHOR
ORIGINAL_ARTICLE
GALLOTANNIN AND FLAVONOID GLYCOSIDES FROM THE STEM BARK OF ACER NEGUNDO (L.)
Gallic acid (1), 1,2-di-O-galloyl- -D-glucopyranoside (2), kaempferol-3-O- -Dglucopyranoside(3), quercetin-3-O- -D-glucopyranoside (4) methyl gallate (5), ethyl gallate(6), isorhamnetin-3-O-rutinoside (7) and quercetin-3-O-rutinoside (8) were isolated for the firsttime from the methanolic extract of the stem bark of Acer negundo (L.). Identification of thesecompounds has been established by physical, chemical and spectral evidence (UV, IR, FAB-MS,1H- and 13C-NMR).
https://bpsa.journals.ekb.eg/article_65470_cf0a3de73c0824eef14c9e893ea92fd0.pdf
2003-06-30
77
82
10.21608/bfsa.2003.65470
Enaam
Backheet
1
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
ORIGINAL_ARTICLE
FLAVONOIDS FROM ONOPORDON HETERACANTHUM C.A. MEY
Seven flavonoids were isolated viz., 3,6,7,4'-tetramethoxy-5,3'-dihydroxy flavonol, 5,7,4'-trihydroxy-6-methoxy flavone (hispedulin), apigenin, 5,7,3',4'-tetrahydroxy-6-methoxy flavone,luteolin, luteolin-7-O-glucoside, acacetin-7-O-rutinoside, in addition to -sitosterol and -sitosterol glucoside from Onopordon heteracanthum. The structures of these compounds wereestablished through the chemical and spectral studies. All the isolated compounds werereported for the first time from the titled plant, while the compounds 3,6,7,4'-tetramethoxy-5,3'-dihydroxy flavonol, 5,7,3'4'-tetrahydroxy-6-methoxy flavone and acacetin-7-O-rutinoside wereisolated for the first time from the genus Onopordon.
https://bpsa.journals.ekb.eg/article_65472_3114fa82240e47fd799cf2abb0849c68.pdf
2003-06-01
83
89
10.21608/bfsa.2003.65472
S.
Youssef
1
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
ORIGINAL_ARTICLE
PHYTOCHEMICAL STUDY OF IXORA FINLAYSONIANA WALL. EX.G.DON GROWING IN EGYPT
A hydrocarbon alcohol, nonacosanol (1), -amyrin (2) and -sitosterol (3) were isolated from the n-hexane fraction of the methanolic extract of Ixora finlaysoniana. From the chloroform fraction, four compounds were isolated; 3-hydroxyhexan-5-olide (4), protocatechuic acid (5), gallic acid (6) and -sitostreol glucoside (7). In addition to three compounds were isolated form the ethyl acetate fraction; parasorboside (8), D-1-O-methyl-myo-inostiol (9) and galactitol (10). The structures of the isolated compounds were elucidated on the basis of physical, chemical and spectroscopic evidence.
https://bpsa.journals.ekb.eg/article_65473_2d3e0a85fa62ea950bed89352ce88da3.pdf
2003-06-30
91
96
10.21608/bfsa.2003.65473
Faten
Darwish
1
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
AUTHOR
Zedan
Ibraheim
2
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
AUTHOR