Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
DEVELOPMENT AND OPTIMIZATION OF ALBENDAZOLE NANOSUSPENSION AS LOCAL ADJUVANT THERAPY FOR TREATMENT OF ENTEROBIASIS
123
134
EN
Aml
I.
Mekkawy
Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag University, Sohag, Egypt
aml.mekkawy@pharm.sohag.edu.eg
Gihan
Fetih
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt & Assiut International Center of Nanomedicine, Al-Rajhy Liver Hospital, Assiut University, Assiut, Egypt
Mahmoud
EL-Badry
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt & Assiut International Center of Nanomedicine, Al-Rajhy Liver Hospital, Assiut University, Assiut, Egypt
Ayat
Allam
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt & Assiut International Center of Nanomedicine, Al-Rajhy Liver Hospital, Assiut University, Assiut, Egypt
allamayat@yahoo.com
10.21608/bfsa.2020.127397
<em>The aim was to develop an optimized albendazole (ALB) nanosuspension to improve its solubility and therapeutic activity as an adjuvant localized therapy for treatment of the pinworm infection to enhance oral treatment outcomes using Box–Behnken design. ALB-nanosuspensions were successfully prepared by antisolvent sono-precipitation technique considering amount of Lecithin, PVP and ultrasonication time as independent variables. All the formulations were characterized regarding their particle size and PDI (dependent variables).</em><br /> <em>Nanoparticle size was significantly increased by increasing lecithin and PVP concentrations while sonication time showed no influence</em><em>. PDI of the nanosuspension was insignificantly decreased with increasing lecithin concentration and probe-sonication time. Optimum formulation was identified and subjected to solid phase characterization and morphological studies. ALB nanosuspension showed 10 folds increase in solubility over pure albendazole powder. Eventually, we studied the anthelmintic activity of ALB nanosuspension compared to free ALB where nanosuspension treated group showed lesser paralysis and death time than free ALB.</em>
https://bpsa.journals.ekb.eg/article_127397.html
https://bpsa.journals.ekb.eg/article_127397_669942dcb2facf5051b3e94ba87067a8.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND IN-VITRO ANTIDIABETIC ACTIVITY OF AN AYURVEDIC FORMULATION
135
139
EN
S.
Gayatri
Assistant Professor, Sri Ramachandra Faculty of Pharmacy, SRIHER (DU), Porur, Chennai-600116, Tamil Nadu, India
V.
Angel Seslin
Monica
Student, Sri Ramachandra Faculty of Pharmacy, SRIHER (DU), Porur, Chennai-600116, Tamil Nadu, India
10.21608/bfsa.2020.127400
<strong><em>Objective:</em></strong><em> To carry out the preliminary phytochemical analysis and to perform the in-vitro anti diabetic activity for the ayurvedic poly herbal formulation. </em>
<strong><em>Material and Methods:</em></strong><em> Preliminary phytochemical analysis was performed as per the standard procedures. In-vitro anti-diabetic activity was done by two methods namely alpha amylase inhibitory assay and alpha glucosidase inhibitory assay by taking acarbose as a standard drug for the ayurvedic formulation. Statistical analysis is carried out by means of graphpad prism software version 6. </em>
<strong><em>Results and conclusion:</em></strong><em> Preliminary phytochemical analysis showed the presence of alkaloids, glycosides, phytosterols, phenols, flavonoids and terpenoids in the formulation. The poly herbal tablet produced 32.2% inhibition of alpha amylase activity at a concentration of 125 µg/ml and 80.8% inhibition of alpha glucosidase activity at a concentration of 62.5 µg/ml. The present study shows that the selected ayurvedic poly herbal tablets has an anti-diabetic activity comparable with that of allopathic drug - acarbose tablet.</em>
https://bpsa.journals.ekb.eg/article_127400.html
https://bpsa.journals.ekb.eg/article_127400_b690b78f2938fb1346b68853783fc0af.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
PHYTOCHEMICAL AND BIOLOGICAL STUDY OF CENTAUREA CINERARIA L. CULTIVATED IN SYRIA
141
147
EN
R.
Joujeh
Department of Plant Biology, Damascus University, Damascus, Syria
10.21608/bfsa.2020.127401
<em>In the current study, </em><em>C. cineraria plant was subjected to chemical and biological study. The plant </em><em>extract was screened for its phytochemical constituents, its quantitative content of phenolics, and its activity in scavenging free radical (DPPH). The </em><em>plant was subjected </em><em>to a biological study, which includes investigating the potential cytotoxic effect of the plant towards human erythrocytes membranes, and the ability of the plant to protect erythrocytes against </em><em>oxidative hemolysis. </em><em>The results indicated that the extract did not contain saponins. Our study also showed that the extract contains a </em><em>good content of phenolics (41.12±0.6) mg GAE/g, and has good DPPH radical scavenging</em><em>activity (79.96%±1.14).</em><em> The results showed that the extract does not have a toxic effect on erythrocytes membranes, and it is highly effective in protecting the membranes from oxidative damage (IC<sub>50</sub> value was 408.86 μg/ml). We hope that future studies will focus on the pharmaceutical aspects of this plant, and will not be considered as ornamental plant only.</em><em><br /></em>
https://bpsa.journals.ekb.eg/article_127401.html
https://bpsa.journals.ekb.eg/article_127401_d4fc68835d9a7fbed7cfd44d260ce85f.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
ARISTOLOCHIC ACID I DETERMINATION IN ARISTOLOCHIA CLEMATITIS L. RAW MATERIALS BY HPLC METHOD
149
155
EN
Lala
Pohodina
0000-0003-4547-2776
Department of Chemistry of Natural Compounds and Nutritiology, National University of Pharmacy, Kharkiv, Ukraine
pogodinalala@gmail.com
Nadiia
Burda
0000-0002-7435-5731
Department of Chemistry of Natural Compounds and Nutritiology, National University of Pharmacy, Kharkiv, Ukraine
nadegdaburda@ukr.net
Viktoriia
Kyslychenko
0000-0002-0851-209X
Department of Chemistry of Natural Compounds and Nutritiology, National University of Pharmacy, Kharkiv, Ukraine
cncvc55@gmail.com
Larisa
Andryukova
0000-0002-8705-9042
Department of Industrial Pharmacy and Economics, National University of Pharmacy, Kharkiv, Ukraine
larisanikand@gmail.com
Artur
Martynov
0000-0003-1428-0085
Mechnikov Institute of Microbiology and Immunology, National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine
imiamn@gmail.com
10.21608/bfsa.2020.127407
<em>Aristolochia clematitis has long ago been applied in Asian traditional medicine and demonstrates multidirectional pharmacological activity. Contemporary research has confirmed its expressed antimicrobial activity. </em><br /> <em>Using high performance liquid chromatography (HPLC) we ascertained and determined the content of aristolochic acid 1 in herb and roots of Aristolochia clematitis collected within the blooming period in Kharkiv and Khmelnytskyi Regions, Ukraine. As aristolochic acid I causes nephrotoxic activity of plants, particularly, Aristolochia clematitis, it is quite mandatory to control its content in raw materials. The content of aristolochic acid I in Aristolochia clematitis herb was 0.11±0.01%, in roots its content was 0.14±0.01%. Thus, issuing from the results of our experiment, we concluded that Aristolochia clematitis raw materials may be treated as feasible for the development of drugs, but only for external application. The results of our study may be used for the development of Aristolochia clematitis raw materials standardization parameters and safe application.</em>
https://bpsa.journals.ekb.eg/article_127407.html
https://bpsa.journals.ekb.eg/article_127407_3d9f7ae843bff6e86b1c08a0602b7477.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
ANTI-INFLAMMATORY ACTIVITY OF THE FOOD PLANT CALLIGONUM POLYGONOIDES L. FLAVONOIDS TARGETING NF-κB
157
164
EN
Ahmed
M.
Zaher
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
Mai
A. M.
Ahmed
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
mai179@yahoo.com
Nesma
M.
Mohamed
0000-0001-8357-6942
Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
nesma.mohamed269@pharm.aun.edu.eg
10.21608/bfsa.2020.127408
<em>Calligonum polygonoides L. subsp. comosum (Polygonaceae) is a wild shrub that grows on the sandy deserts of various regions in North Africa, Western Asia and Southern Europe. In some of Asian countries, the flower buds and young branches of the plant are used to prepare traditional food dishes. Also, different organs of C. polygonoides L. are used traditionally in treatment of stomach diseases. The isolation of anti-inflammatory lead compounds was performed by using different normal silica gel, reversed phase C-18, and sephadex LH-20 open columns. The identification of the isolated compounds was done using NMR spectral techniques. The isolated compounds are of different flavonoid classes; two flavonol glycosides; kaempferol-3-O-β-D-glucuronide and mequilianin, one flavan-3-ol; catechin, and one dihydro-flavonol; taxifolin. The methanol extract and ethyl acetate fraction of Calligonum polygonoides L. subsp. comosum exhibited anti-inflammatory activity against NF-κB translocation pathway on HEK293 cells. All of them were active against NF-κB translocation, and previously isolated from the plant under study. Kaempferol-3-O-β-D-glucuronide, a flavonoid glycoside demonstrated the most potent NF-κB inhibition in comparing with other flavonoids; taxifolin, catechin, and mequilianin. Therefore, the food plant aerial parts of C. polygonoides are rich in nutraceuticals (flavonoids and flavonoid glycosides) that can be utilized in the treatment of vascular inflammation.</em>
https://bpsa.journals.ekb.eg/article_127408.html
https://bpsa.journals.ekb.eg/article_127408_246ec512bc3ea4711f255287667b1c96.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
REAL-WORLD SAFETY AND EFFICACY OF TICAGRELOR IN PATIENTS WITH ST SEGMENT ELEVATION MYOCARDIAL INFARCTION AT ASSIUT UNIVERSITY HEART HOSPITAL
165
176
EN
Huda
AlSayed
Mohammed
Clinical Pharmacist, Coronary Care Unit, Assiut University Heart Hospital, Assiut, Egypt
huda_89@hotmail.com
Mohamed
Aboel-Kassem F.
Abdelmegid
Department of Cardiovascular Medicine, Faculty of Medicine, Assiut University, Assiut, Egypt
aboelkassem1970@yahoo.com
Ahmed
Mostafa
Alsayed
Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt
ahmed.mostafa70020@yahoo.com
Mostafa
A. Sayed
Ali
Department of Clinical Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
10.21608/bfsa.2020.127409
<strong><em>Objectives:</em></strong><em> The new generation antiplatelets are generally recommended by the current US and European guidelines. This article aimed to address the efficacy and safety of using ticagrelor in patients with ST-elevated acute coronary syndromes.</em><br /> <strong><em>Methods:</em></strong><em> This was a prospective observational study on 120 patients admitted to the Cardiology Care Unit of Assiut University Hospital with ST-segment elevation acute coronary syndrome, who were admitted for urgent revascularization and received aspirin and the P2Y12 antagonist ticagrelor. The patients were followed for three months between May 2018 and September 2019. The primary efficacy end point was the incidence of major adverse cardiovascular events (MACE).</em><br /> <strong><em>Results:</em></strong><em> At three months, the primary efficacy end point, a composite of cardiovascular death, myocardial infarction, or stroke - occurred in 11/120 (9.2%) patients. The primary safety end point, the incidence of major bleeding, was observed in two patients (1.7%) with no fatal bleedings. Minor and minimal bleeding occurred in 2.5% and 20.8% of patients, respectively. Other efficacy endpoint was myocardial infarction 4.2% and stent thrombosis (1.7%). Severe dyspnea found in 5.0% patients and led to 1.7% ticagrelor discontinuation. Multivariate regression analysis revealed no association between bleeding and patients’ age, sex nor IV administration of glycoprotein IIb/IIIa inhibitor.</em><br /> <strong><em>Conclusion:</em></strong><em> The MACE with ticagrelor was higher than previously reported in most studies. Dyspnea rate was higher and more pronounced than bleeding rate. The majority of bleeding cases was minimal with no fatal major bleeding and no bleeding led to comorbidity.</em>
https://bpsa.journals.ekb.eg/article_127409.html
https://bpsa.journals.ekb.eg/article_127409_03fd96d9896cfcddc9a5683fef925f67.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
KNOWLEDGE AND AWARENESS OF THE NOVEL CORONAVIRUS DISEASE (COVID-19) PANDEMIC AMONG EGYPTIAN POPULATION
177
189
EN
Osama
Mohamed
Ali
Clinical Pharmacy Program Student, Faculty of Pharmacy, Assiut University, Assiut, Egypt
Abdurahman
Salah
Mohamed
Clinical Pharmacy Program Student, Faculty of Pharmacy, Assiut University, Assiut, Egypt
apood7932869@gmail.com
Esmael
Ibrahim
Mohamed
Clinical Pharmacy Program Student, Faculty of Pharmacy, Assiut University, Assiut, Egypt
asmaalibrahim@yahoo.com
Sara
Fekry
Abdullah
Clinical Pharmacy Program Student, Faculty of Pharmacy, Assiut University, Assiut, Egypt
sasa.feff99@gmail.com
Sahar
Badr
Hassan
Department of Clinical Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
saharbadr164@yahoo.com
Mohamed
Mahmoud
Abdel-Latif
Department of Clinical Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
m.abdellatif@aun.edu.eg
10.21608/bfsa.2020.127413
<em>On 11 March 2020, WHO declared the novel Coronavirus disease 2019 (COVID-19) a pandemic disease worldwide. With the rapid spread of COVID-19, Egyptians are at a high risk of being infected, as it is the most populous country in the Middle East and North Africa region. The aim of the present study was to assess the knowledge and awareness of Egyptians towards COVID-19. A nationwide cross-sectional study was performed among the general sample of the Egyptian population. One thousand one hundred fifteen individuals participated in the survey with gender distribution of 625 males and 490 females. Descriptive analysis was used to describe the study findings. Most respondents 715 (64.1%) acknowledged that COVID-19 was a serious disease and the majority of them had a good to fair knowledge 1096 (98.3%) about COVID-19. Nasal droplets and touching surfaces were suggested to be the common modes of transmission 1099 (98.6%). Most respondents correctly identified the symptoms of COVID-19 of fever, dry cough and dyspnea. The main sources of information obtained from social media 979 (87.8%), radio-television 611 (54.8%) and Ministry of Health 568 (50.9%). However, only 144 (12.9%) of participants trusted information posted on social media, 605 (54.3%) to some extent while 366 (32.8%) didn't. Despite many participants understood the precautionary measures, but they were unsatisfactory 796 (71.4%) with the public's behavior of obeying the national procedures. The surveyed Egyptians had a good knowledge of COVID-19 and an acceptable understanding of the precautionary measures taken by the government. However, strict national procedures are further needed to enforce such measures to combat the disease.</em>
https://bpsa.journals.ekb.eg/article_127413.html
https://bpsa.journals.ekb.eg/article_127413_5312024b52fe4063d45a2e79bb1deeb0.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
ASSESSMENT OF KNOWLEDGE BELIEVES AND MYTHS REGARDING THE USE OF MASK IN COVID-19 OUTBREAK IN KARACHI PAKISTAN
191
198
EN
Saira
Shahnaz
Department of Pharmacy Practice, Faculty of Pharmacy, Ziauddin University, Karachi, Pakistan
msairakhan1302@gmail.com
Mudassar
Iqbal
Arain
Department of Pharmacy Practice, Faculty of Pharmacy, University of Sindh Jamshoro, Pakistan
mudassarpk1302@gmail.com
Khurram
Anwar
Isra Dental College, Hyderabad, Pakistan
khurramanwar1@gmail.com
Ramesha
Anwar
Faculty of Pharmacy, University of Sindh, Jamshoro, Pakistan
rameshaanwar1@gmail.com
10.21608/bfsa.2020.127414
<strong><em>Objective:</em></strong><em> To assess the general perspective of population their believes and myths regarding the pandemic outbreak and use of mask as precautionary measures. </em>
<strong><em>Methods:</em></strong><em> A Cross-sectional observational study conducted from January-June 2020, among the general population of Karachi. Total 470 participant age range of 18-60 years were included in the study. Data was analyzed by SPSS version 21.0, for descriptive statistics. Correlation between variables was assess by Kruskal Wallis Test, spearman rank correlation was established to assess the significant correlation between Knowledge, myths and practices. </em>
<strong><em>Result:</em></strong><em> Out of 470 respondents, 74.31% were male and 25.69% of female. More than half of respondents aged between 51 to 70 years i.e. 53.92%. While 21.76% respondents were categorized in illiterate category. The mean rank score for knowledge, myths and practices were 217.95±2.1, myths score was 236.07±2.1, 241.12±2.1 respectively. The spearman rank correlation was established between knowledge myths and practices, a significant correlation between was established by Knowledge-myths (r= 0.0176, p= 0.015), knowledge-practices (r= 0.0174, p= 0.001) however myths-practices (r= 0.0170, p < 0.001). Believes and myths related to the mask was concerned i.e. 56.1 were using N-95 masks, 89% respondents reused the masks, 59.1% used the mask between 2 to 5 times. Wearing mask is not a precautionary measure was mainly found as a myth in respondents i.e. 78.7%. 67.2% respondents had myths about unnecessary use of mask. The study highlighted the non-optimal behavior and practice with regards to the use of preventive measure including the use of mask in the pandemic outbreak.</em>
https://bpsa.journals.ekb.eg/article_127414.html
https://bpsa.journals.ekb.eg/article_127414_9cc8d8417196c7e83e9dbc0fae619fd1.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
PREDICTORS OF HCV SEROCONVERSION AMONG END-STAGE RENAL DISEASE PATIENTS IN HEMODIALYSIS UNIT
199
208
EN
Fakhara
Bukhari
Department of Pharmacy, Quaid-I-Azam University, Islamabad, Pakistan
drfakhara.bukhari@yahoo.com
Ahmad
Khan
Department of Pharmacy, Quaid-I-Azam University, Islamabad, Pakistan
akhan@qau.edu.pk
Tahir
Mehmood
SNS, NUST, Islamabad, Paksitan
10.21608/bfsa.2020.127415
<strong><em>Background:</em></strong><em> In patients under dialysis, blood borne infections remains one of the mostpublic causes of morbidity, hospitalization and death. Among dialysis patient’s long-term hemodialysis (HD) therapy, frequency of blood transfusions and multiple blood transfusions enhance the risk of acquiring blood-borne infections. </em>
<em>Objective: The aim of the study was to identify predictors for HCV seroconversion in HD patients. </em>
<strong><em>Method:</em></strong><em> Prospective observational study was conducted on 173 Chronic kidney disease (CKD) patients undergoing HD. Anti HCV antibody testing was accomplished at the start of study and every 2-3 months subsequently. To identify seroconversion, patients who were sero-negative for HCV were monitored.</em>
<strong><em>Results:</em></strong><em> Prospective follow-up exposed an occurrence of seroconversion of 12.1% during study duration. On multivariate analysis, both increase in the duration on hemodialysis besides attending more than one centre for HD were significantly associated with anti-HCV positivity (p < 0.05, OR 0.185), (p < 0.05, OR 4.52) respectively. Risk factors blood transfusion and AST >40 IU/L also shows significant association for seroconversion (p < 0.05). </em>
<strong><em>Conclusion:</em></strong><em> It is concluded that increase post dialysis incidence of HCV occurs as specific guidelines for HCV infected patients were not followed. Regular surveillance for HCV infection helps dramatically in decreasing the spread of HCV in hemodialysis unit.</em>
https://bpsa.journals.ekb.eg/article_127415.html
https://bpsa.journals.ekb.eg/article_127415_d64b8579f93abca653915e19eae02f8f.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
THE PRESCRIBING PATTERN OF ANTI-ASTHMATIC DRUGS FOR THE TREATMENT OF ASTHMA, AT DIFFERENT SETTINGS OF KARACHI, PAKISTAN
209
215
EN
Saira
Shahnaz
Department of Pharmacy Practice, Ziauddin University, Karachi, Pakistan
msairakhan1302@gmail.com
10.21608/bfsa.2020.127416
<strong><em>Introduction:</em></strong><em> Asthma is a common chronic inflammatory disorder of respiratory airways with high growing prevalence around the globe.</em>
<strong><em>Objectives:</em></strong><em> To evaluate the current prescribing practice and analyze the prescription for rational use of drugs of asthma by comparing with international guidelines of GINA (Global Initiative for Asthma.</em>
<strong><em>Methodology:</em></strong><em> A cross-sectional study was conducted among the patients with confirmed diagnosis of asthma for the period of eight months at different setups of Karachi. The prescription and patients record files were evaluated for prescribing pattern and disease profile of asthma. </em>
<strong><em>Results:</em></strong><em> study concluded 73.5% (n= 735) patients were male, while 26.5% (n= 265) patients were females, 42% (n= 105) patients were from inpatients setting of public sector hospitals, 26.8% (n= 67) from private sector hospital and 50% (n= 250) patients were from private clinics, while 58% (n= 145) were from out-patients departments of public sector hospitals. The inhaled corticosteroids were prescribed to almost more than half of the enrolled patients either monotherapy or combined drugs therapies 44.1% (n= 64), medication of class long acting beta agonists to 22.7% (n= 33) patinets, 17.2% (n= 25) patients were prescribed with leukotriene inhibiters. </em>
<strong><em>Conclusion:</em></strong><em> The asthma treatment guidelines suggests the prescribing of corticosteroids and long acting beta agonists in combinations (LABA’s+ICS), that was observed in current study as successful therapy in two drug combinations therapies. Fluticasone+Salmeterol combination was observed as successful therapy.</em>
https://bpsa.journals.ekb.eg/article_127416.html
https://bpsa.journals.ekb.eg/article_127416_668c60334523ffcd4f6094d30409bef0.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
PROSPECTS FOR USING MITOGEN-ACTIVATED PROTEIN KINASES ERK1/2 AND p38 OF NERVE TISSUE PROGENITORS AS PHARMACOLOGICAL TARGETS FOR THE TREATMENT OF NEURODEGENERATION CAUSED BY ALCOHOL
217
224
EN
Gleb
N.
Zyuz'kov
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia, 3 Lenin Prospect, Tomsk, 634028, Russia
Larisa
A.
Miroshnichenko
0000-0002-3684-5510
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia, 3 Lenin Prospect, Tomsk, 634028, Russia
laarmi@yandex.ru
Elena
V.
Simanina
0000-0003-1890-6946
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia, 3 Lenin Prospect, Tomsk, 634028, Russia
elena_simanina@mail.ru
Tatyana
Yu.
Polyakova
0000-0003-0971-7844
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia, 3 Lenin Prospect, Tomsk, 634028, Russia
tasha93@mail.ru
10.21608/bfsa.2020.127417
<em>We studied the possibility of using ERK1/2 inhibitor and p38 inhibitor to modulate the functions of nerve tissue precursor cells in ethanol-induced neurodegeneration. It has been found that in chronic alcoholization of mice both these pharmacological substances are able to increase proliferative activity of neural stem cells (NSC). In addition, the blockade of ERK1/2 initiates the progression of the cell cycle of the neuronal-committed progenitors (NCP, clonogenic PSA-NCAM + cells). This reduces the differentiation intensity of NSC and clonogenic PSA-NCAM + cells under the influence of the ERK1/2 inhibitor and such in NSC under the influence of p38 inhibitor. The fundamental difference between the functions of ERK1/2 and p38 in the regulation of the NSC and NCP cell cycle in optimal living conditions and in ethanol-induced neurodegeneration does not allow to draw unambiguous conclusions about the prospect of using these protein kinases as pharmacological targets of drugs for the therapy of alcoholic encephalopathy.</em>
https://bpsa.journals.ekb.eg/article_127417.html
https://bpsa.journals.ekb.eg/article_127417_b068008068d4fdb15622facc7d967606.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
PHENOTYPING CYP3A4/5 USING AN ENDOGENOUS BIOMARKER IN CHILDREN WITH DOWN SYNDROME
225
235
EN
Zoheir
A.
Damanhouri
Department of Pharmacology, Faculty of Medicine, King Abdulaziz University (KAU), Saudi Arabia
Ahmed
S.
Ali
Department of Pharmacology, Faculty of Medicine, King Abdulaziz University (KAU), Saudi Arabia & Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt
Jumana
Y.
AlAama
Princess Al-Jawhara Center of Excellence in Research of Hereditary Disorders, King Abdulaziz University (KAU), Saudi Arabia
Fuad
Y.
Almaweri
Department of Pharmacology, Faculty of Medicine, King Abdulaziz University (KAU), Saudi Arabia
fuadalmaweri@hotmail.com
Alaa
M.
Khedr
Departmentt of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University (KAU), Saudi Arabia
akhedr@kau.edu.sa
Lateef
M.
Khan
Department of Pharmacology, Faculty of Medicine, King Abdulaziz University (KAU), Saudi Arabia
lmkhan00@hotmail.com
10.21608/bfsa.2020.127418
<strong><em>Background: </em></strong><em>Down syndrome (DS); a common chromosomal abnormality in humans can affect multiple organ systems. DS individuals usually use a wide range of medications. There is a gap in knowledge about the extent of contribution of CYP3A4/5 in altered clinical response to medications in DS children. </em><br /><strong><em>Objectives:</em></strong><em> Phenotyping of CYP3A4/5 in DS children using. the ratio of 4β-hydroxycholesterol / Cholesterol (4β-OHC/C) as an endogenous biomarker for CYP3A4/5 activity. </em><br /><strong><em>Method:</em></strong><em> The study was an observational case control study, conducted in the DS clinic, King Abdul-Aziz University Hospital. Blood samples were taken for thyroid and liver function test by automated immunoassay procedure and analysis of cholesterol and 4β-hydroxycholesterol by gas chromatography. </em><br /><strong><em>Results:</em></strong><em> 16 DS and 29 non-DS children were enrolled (1-12 Y). Children with DS showed a lower median 4β-OHC/C molar ratio of 0.19×10<sup>-4 </sup>compared to 0.45×10<sup>-4 </sup>in the control group and with interquartile range (IQR) 0.17, 0.36 respectively (p < 0.001 Mann Whitney U test). DS children also showed an abnormality in liver enzymes and hypercholesteremia.</em><br /><strong><em>Conclusion:</em></strong><em> Children with DS had about two-fold lower CYP3A4/5 activity compared to children without DS. More studies to confirm these observations are required, however, drugs should be used cautiously in DS children.</em>
https://bpsa.journals.ekb.eg/article_127418.html
https://bpsa.journals.ekb.eg/article_127418_77c235e507b16f759c1263df8f6ad0d6.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
CIRCULATING PATTERN OF RESPIRATORY VIRUSES, IN EGYPT, SEASON 2013-2014
237
253
EN
Wael
H.
Roshdy
0000-0001-8412-3128
PhD in Biochemistry and Molecular Virology, Head of Tissue Culture and Virus Isolation, Central Public Health Laboratories, CPHL, Ministry of Health, Cairo 11613, Egypt
waelhamedroshdy@yahoo.com
Amel
M.
Naguib
MSc in Clinical Pathology, Head of Virology Directorate, Central Public Health Laboratories, CPHL, Ministry of Health, Cairo 11613, Egypt
amelnaguib11@gmail.com
Ibrahim
Elsayed
Department of Biochemistry, Faculty of Science, Kafr Elsheikh University, Kafr Elsheikh 33784, Egypt
ibrahimelsayed@yahoo.com
Yara
I.
Shamikh
0000-0001-7535-459X
Department of Microbiology and Immunology, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, Egypt & Consultant, Virology Department, Egypt Centre for Research and Regenerative Medicine, ECRRM, Cairo 11517, Egypt
yara.shamikh@hotmail.com
10.21608/bfsa.2020.127419
<em>Respiratory viruses causes lower and upper respiratory tract infections; LRTIs and URTIs. These infections are from the predominant causes of infections, especially in children and infants.</em>
<strong><em>Objective</em></strong><em>: This study, provides the evidence of using modern techniques as the multiplex PCR method, as a helpful tool</em><em> in identifying</em><em> the circulating pattern of respiratory viruses, in Egypt, thus this could help in identifying and inventing new treatment and vaccines.</em>
<strong><em>Subjects and methods:</em></strong><em> A number of 237 nasopharyngeal aspirates, were collected between December 2013 to November 2014, from 2 Egyptian fever hospitals, classified into 3 groups; group 1; 37 normal persons, group 2; 100 patients with LRTIs or pneumatic group and group 3; 100 patients with URTIs or common cold group. The results were then assayed using multiplex RT-PCR, in the Central Public Health Laboratories, CPHL, Ministry of Health, Cairo, Egypt.</em>
<strong><em>Results:</em></strong><em> Respiratory viruses, were detected in 152 cases, where the human rhinovirus was the most prominent; 15.6%, followed by the respiratory syncytial virus, 10.5%, metapneumovirus; 13%, influenza-A (Flu-A); 6.3%, adenovirus (AdV); 5.9%, influenza-B (Flu-B); 5.4%, parainfluenza-3 (PIV-3); 4% and a percentage of 0.42% infection for parainfluenza-1 (PIV-1), parainfluenza-2 (PIV-2) and human bocavirus (HBoV). No infections with parainfluenza-4, enterovirus nor the coronaviruses were detected. Both season and age of the patients, affect the detection rate of the analysis.</em>
https://bpsa.journals.ekb.eg/article_127419.html
https://bpsa.journals.ekb.eg/article_127419_4e2511ac6ef16a5ba9fd65c3bc7a602a.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
VIRULENCE DETERMINANTS OF ESCHERICHIA COLI STRAINS ISOLATED FROM SURGICAL SITE INFECTIONS AT SELECTED HOSPITALS IN SYRIA
255
264
EN
N.
Daoud
Department of Biochemistry and Microbiology, Faculty of Pharmacy, Damascus University, Syria & Department of Life Science, Faculty of Dentistry, Damascus University, Syria
A.
Abou Sulaiman
Department of Periodontology, Faculty of Dentistry, Damascus University, Syria
K.
H.
Al Kwatli
Department of Biochemistry and Microbiology, Faculty of Pharmacy, Damascus University, Syria
10.21608/bfsa.2020.127420
<em>Escherichia coli is one of the most commonly isolated pathogens from surgical site infections (SSIs) that accounts for significant morbidity and mortality, especially with high virulent strains. The purpose of this study was to evaluate the virulence determinants of E. coli strains isolated from surgical sections. 51 E. coli isolates were assessed for biofilm formation, mannose-sensitive or -resistant hemagglutination, capsule, hemolysin and siderophores production. Antibiotic susceptibility against 14 common antibiotics was performed. As a result, 25 strains displayed 5 virulence factors and multiple drug resistance towards more than 11 antibiotics. DNA and plasmids were extracted from the 25 virulent isolates. PCR was used to investigate virulence genes on DNA encoding adhesins (fimH-1, mrkD), hemolysin (hlyA), siderophores (entB: enterobactin, iutA: aerobactin, irp-1: yersiniabactin- which was encoded on (HPI) high-pathogenicity island). MrkD, iutA and hlyA genes were also screened on extracted plasmids. The most common virulence genes were iutA (25/100%), mrkD (24/96%), entB (23/92%) and fimH-1 (21/84%). Irp1 was found at moderate rates (15 /60%) and at lower prevalence, was gene hlyA (2 / 8%). Plasmids were found in 16/25 strains. MrkD and iutA were present in 10 /16 plasmids, whereas none of them harbored hlyA. In conclusion, most E. coli isolates harbored high frequencies of (fimH1, mrkD, entB and iutA) which seem to be at the basis of pathogenicity. However, some strains, which carry HPI and have virulence plasmids can account for even more real threat if they spread among other Enterobacteriaceae members in surgical sections.</em>
https://bpsa.journals.ekb.eg/article_127420.html
https://bpsa.journals.ekb.eg/article_127420_6bf0a88b17fa5ce705634f7ee73fe552.pdf
Assiut University, Faculty of Pharmacy
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
3009-7703
43
2
2020
12
01
ANTIBIOTIC RESISTANCE OF ACINETOBACTER BAUMANNII: AN URGENT NEED FOR NEW THERAPY AND INFECTION CONTROL
265
279
EN
Randa
Abd-ElNasser
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt & Ministry of Health and Population, Assiut, Egypt
Mona
Hussein
Abdel-Rahim
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
Manal
Ahmed
Mahmoud
Department of Chest Diseases, Faculty of Medicine, Assiut University, Assiut, Egypt
Amal
Ahmed
Elkhawaga
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
Khaled
Mohamed
Hassnein
Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
10.21608/bfsa.2020.127991
<em>Acinetobacter baumannii (A. baumannii) infections became an emerging health concern in hospitals across the globe and are often related to nosocomial infections with poorer clinical outcomes in patients with prolonged hospital stay. Management of infections involves prompt identification of the infecting strain, isolating the source of infection, and proper choice of antibiotic regimen. However, resistance to first-line antimicrobial drugs, combined with a scarcity of equally effective alternatives, complicates the treatment of multidrug-resistant (MDR) A. baumannii. Presently, MDR A. baumannii may be a serious health concern in hospitals and long-term care facilities accounting for up to 20% of infections in intensive care units, and 7% of infections in patients who are physically connected to medical equipments. Immediate and sustained prevention efforts are needed to control the speed of incidence. Antibiotic use is largely under-regulated in Egypt leading to the emergence of resistant isolates. This review describes genetic markers and other factors that influence the incidence of MDR A. baumannii. Current and emerging treatments as well as infection control strategies are discussed.</em>
https://bpsa.journals.ekb.eg/article_127991.html
https://bpsa.journals.ekb.eg/article_127991_e176221cf7954e541f8db6b5fc2f8c43.pdf