2024-03-29T13:07:05Z
https://bpsa.journals.ekb.eg/?_action=export&rf=summon&issue=9487
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
1110-0052
2014
37
2
PHYTOCHEMICAL INVESTIGATION OF THE AERIAL PARTS OF CENTAURIUM SPICATUM WITH HEPATOPROTECTIVE AND mRNA ENZYMATIC INHIBITION ACTIVITIES
Ahmed
Allam
Mohamed
El-Shanawany
Enaam
Backheet
Alaa
Nafady
Five compounds (1-5) were firstly reported from the genus Centaurium family Gentianaceae. They were identified as Lisianthoside 1, Secoxyloganin 2, Secologanin dimethyl acetal 3, 1,8-Dihydroxy-3,5,6,7-tetramethoxyxanthone (Demethyleustomin) 4 and 1-Hydroxy3,5,6,7,8-pentamethoxyxanthone (Eustomin) 5. Structure elucidation was carried out with support of chemical and spectral analysis including 1D and 2D NMR experiments. All the isolated compounds showed promising antioxidant activity, liver microsomal enzymes reducing activity and CYP3A4 mRNA inhibition activity in the HepG2 cell line.
2014
12
31
65
76
https://bpsa.journals.ekb.eg/article_65789_64e22b97df0843ea6cdbf11a4c992c43.pdf
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
1110-0052
2014
37
2
EVALUATION OF POTENTIAL PROTECTIVE EFFECTS OF CURCUMIN VERSUS METFORMIN IN EXPERIMENTALLY-INDUCED METABOLIC SYNDROME IN RATS
Abdel-Halim
Afifi
Hussein
El-Bitar
Marwa
Ahmed
Metabolic syndrome (MetSyn) is the clustering of various interrelated risk factors of metabolic origin that increased incidence of cardiovascular diseases and type 2 diabetes. In this study the effectiveness of curcumin was evaluated in comparison with metformin in fructose-induced MetSyn disease in rats. Fructose was fed (10% solution in drinking water) for 8 weeks during which groups of rats were administered once daily vehicle (2% carboxy methyl cellulose), curcumin (40 and 80 mg/kg), metformin (100 and 200 mg/kg), their combinations and compared with group received tap water instead of fructose. The results revealed that induction of MetSyn was associated with glucose intolerance, insulin resistance alongside with increased weights of body and visceral fats. This was accompanied with an elevation of arterial blood pressure. Meanwhile, it caused disturbances in lipid profile (triglyceride, total cholesterol, HDL-C and LDL-C) and both oxidative stress (malondialdehyde, 8-iso-PGF2 α and superoxide dismutase) and inflammatory status (tumor necrosis factorα , interleukin-6, Creactive protein and adiponectin) parameters. Each of curcumin and metformin significantly prevents, to variable extents, the progression of most of these signs of MetSyn. Furthermore, the efficacy of each of the two drugs in question was significantly augmented upon their concurrent administration. These protective effects of the two drugs under investigation presumably may be relevant to their ability to reduce the oxidative stress and to ameliorate the inflammatory processes.
2014
12
31
77
90
https://bpsa.journals.ekb.eg/article_65790_1cfd89c3fcebbbc9e1ceaa1fb5f50e3a.pdf
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
1110-0052
2014
37
2
LEPTIN INTERRELATION BETWEEN ADIPOGENESIS AND ANGIOGENESIS WITH RESPECT TO THE INFLAMMATORY STATUS AND SEVERITY OF THE UNDERLYING NON-ALCOHOLIC FATTY LIVER DISEASES IN OBESE ALBINO RATS
Maiiada
Nazmy
Manal
Abdel-Ghany
The present study aims to offer more reliable non-invasive markers for the distinction between pure fatty change and steatohepatitis. Sixty male albino rats, equally divided into 4 groups (15 rats/group). Group I acted as normal control were fed standard diet. Groups II, III, IV were fed high fat diet for 4, 8, 12 weeks respectively. High fat diet caused sustained hyperglycemia, hyperinsulinemia and insulin resistance. It also induced significant histopathological and biochemical changes in liver enzymes, lipid profile and lipid peroxidation. It significantly increased both serum and tissue levels of leptin, vascular endothelial growth factor, tumor necrosis factor- alpha and interleukin-6. All tested parameters were significantly correlated with histological scoring. Suggested parameters, based on their involvement as key players in disease pathogenesis, may be useful as non-invasive diagnostic tools for obesity-related non-alcoholic fatty liver diseases.
2014
12
31
91
103
https://bpsa.journals.ekb.eg/article_65791_1ba65ba21b90291d26aecfefca66a8d1.pdf
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
1110-0052
2014
37
2
STIMULATION OF MELANOGENESIS BY POLYPHENOLIC COMPOUNDS FROM CITHAREXYLLUM QUADRANGULARE IN B16F1 MURINE MELANOMA CELLS
Ahmed
Allam
Two polyphenolic compounds; 1,3,6-tri-O-galloyl-β-D-glucopyranose (TGG) (1) and methyl gallate (2) have been isolated from the aerial parts of Citharexylum quadrangulare Jacq. They were isolated for the first time from the genus Citharexylum and their structures have been established on the basis of spectroscopic methods in addition to comparison with literature data. The stimulatory activity of the isolated compounds was investigated on melanin synthesis where they stimulate the intracellular synthesis of melanin in murine melanoma B16F1 cells in cultured cell lines. The melanin content was dramatically increased by these compounds in a dose dependent manner and at nontoxic concentrations where IC50 of TGG was 20.4 µM and that of methyl gallate was 16.1 µM. Moreover, the isolated compounds were tested for cytotoxic activity where IC50 of compounds (1) & (2) were 1.5 and 10.8 µM, respectively. Also, they exhibited higher radical scavenging activity than ascorbic acid in DPPH assay system with IC50 of 1.5 and 10.8 µM for compounds (1) & (2) respectively. Dramatic stimulatory effect of the isolated compounds on melanogenesis might be applied to various conditions of hypopigmentation-related disorders like Vitiligo as an adjunctive therapy in addition to its safely use as an antioxidant. The obtained results indicated that these active compounds could be used safely in cosmetic purposes.
2014
12
31
105
115
https://bpsa.journals.ekb.eg/article_65793_fa3f066322b1bcc360f017f95e6cc7a5.pdf
Bulletin of Pharmaceutical Sciences Assiut University
1110-0052
1110-0052
2014
37
2
REVIEW ARTICLE CHITOSAN MICROSPHERES AS POTENTIAL CARRIERS FOR COLON TARGETING
Hytham
Gadalla
A.
El-Sayed
Fergany
M.
I.
El-Gibaly
Ghareb
Soliman
Chitosan microspheres have been in the focus of increasing interest as polymeric drug carriers due to their appealing properties such as biocompatibility, biodegradability, low toxicity, mucoadhesion and relatively low cost of production. Gel formation can be obtained by interactions of chitosan with low molecular weight counter-ions such as polyphosphates. However, one drawback of using this natural polysaccharide for oral controlled release dosage forms is its fast dissolution rate in the stomach. Since chitosan is positively charged at low pH values (below its pKa value), it spontaneously associates with polyanions to form polyelectrolyte complexes (PEC). These PEC exhibit favorable physicochemical properties with preservation of chitosan's biocompatible characteristics. These complexes are therefore good candidates for the design of colon-targeted dosage forms. Various techniques are used for preparing chitosan microspheres which have been reviewed. This review also includes factors that affect the release characteristics of drugs from chitosan microspheres.
2014
12
31
117
127
https://bpsa.journals.ekb.eg/article_65794_827a71c613b519412cd343c95d48571d.pdf