ALKALOIDS OF HYOSCYAMUS MUTICUS TISSUE CULTURES

Document Type : Original Article

Authors

Department of Pharmacognosy, Faculty of Pharmacy, Tanta University, Tanta, Egypt

Abstract

Callus cultures were developed from Hyoscyamus muticus seeds and stem or Leaf midrib. The culture grows and maintains well in a number of culture media. Alkaloids were produced in all cultures. Chromatographic investigation of the alkaloid composition revealed the presence of hyoscyamine and scopolamine, together with an unidentified alkaloid. Quantitative estimation of the total alkaloids was done by the acid-dye complex spectrophotometric technique.