A convenient and specific spectrophotometric determination of stilbestrol and stilbestrol dipropionate is developed by interaction with 2,3,5-triphenyltetrazolium chloride at 50°C for 45 minutes and subsequent measurement of the color formed at 485 nm. Evidence is provided to account for the significance of extended conjugation of 4,4'-stilbenediol derivatives to induce this color reaction. Ideal adherence of color absorption to Beer's law permitted accurate and precise determination of stilbestrol and stilbestrol dipropionate pure forms over a range of 2-22 mcg stilbestrol/ml. Application of the tetrazolium, reaction to the analysis of stilbestrol dipropionate oily dosage forms could be satisfactorily effected without prior hydrolytic or extraction processes. This offers a fundamental achievement for utility of the proposed procedure in automated analysis.
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