EFFECT OF UV-C STRESS ON TOTAL PHENOLICS, TOTAL FLAVONOIDS AND KHELLIN CONTENTS IN AMMI VISNAGA (L.) LAM. FRUITS

Document Type : Original Article

Authors

1 Pharmacognosy Department, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

2 Student at Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

3 Pharmacognosy Department, Faculty of Pharmacy, Fayoum University, Fayoum, 63514, Egypt

Abstract

Ammi visnaga fruits (L.) Lam., is widely used for its antispasmodic and vasodilating effects. These effects are attributed to its main content of khellin. In our research, we studied the effect of UV-C stress on the metabolite production of the fruits. Fruits were subjected to UV-C treatment for ½, 1, 2, 3, 4 and 5 hr The change in the metabolic profile was studied by the quantification of total phenolics, total flavonoids and khellin in the methanolic extracts of the fruits applied to stress and extracts of control fruits. Total phenolics and total flavonoids were quantified colorimetrically using appropriate reagent for each. Folin-Ciocalteau reagent was applied for the quantification of total phenolics while 2% AlCl3 solution was applied for the quantification of flavonoids.  On the other hand, khellin was quantified by HPLC analysis. Our results showed that the concentrations of total phenolics, total flavonoids and khellin contents of the fruit extracts are significantly increased by exposing the fruits to UV-C stress with maximum production at 1 and 2 hr.

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