THE INHIBITORY EFFECT OF DOUM PALM (HYPHAENE THEBAICA L. MART.) LEAVES EXTRACT ON α-GLUCOSIDASE ACTIVITY

Document Type : Original Article

Authors

1 Pharmacognosy and Natural Products Department, Faculty of Pharmacy, Menoufia University, Shibin Elkom 32511, Egypt

2 Pharmacognosy Department, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

3 Pharmacology Department, Faculty of Medicine, Assiut University, Assiut 71526, Egypt

Abstract

Background: Hyphaene thebaica L. Mart. (Doum-palm), rich in total phenolics content, is known for its medicinal value in the treatment of several health conditions, such as hypertension, and diabetes mellitus. Aim of the Study: To investigate the hypoglycaemic activity of the dichloromethane, ethyl acetate, and aqueous fractions from doum palm leaves. Then, to characterize the metabolic profile of the most active fraction by LC-HR-MS/MS analysis to detect the responsible metabolites for this activity. Material and Methods: The present study examined the in vitro inhibitory effect of the extract fractions from Doum Palm Leaves at concentrations ranging from 7.81 to 1000.00 μg/ml on α-glucosidase activity, an enzyme responsible for carbohydrate-hydrolysis to monosaccharides and intestinal glucose absorption. Metabolic profiling for the dichloromethane fraction was obtained with LC-HR-MS/MS. Results: The dichloromethane (DCM) fraction inhibited α-glucosidase activity in vitro with an IC50 of 52.40 μg/ml. Twenty-three compounds were identified in the DCM fraction by LC-HR-MS/MS analysis. Most of them were reported for their potential antidiabetic activity. Nevertheless, the III-DCM subfraction (IC50 3.79 ± 0.17 μg/ml) and the IV-DCM subfraction (IC50 5.13 ± 0.24 μg/ml) had the best inhibitory activity against α-glucosidase compared with acarbose (IC50 2.33 ± 0.11 μg/ml).  Conclusions: The results support the use of these fractions obtained from Doum palm leaves to effectively inhibit a crucial enzyme linked to type 2 diabetes and suppress carbohydrate absorption from intestine, and thereby reducing the postprandial increase of blood glucose.

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