DETECTION OF CARBAPENEM RESISTANCE AMONG ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE IN CHEST INTENSIVE CARE UNIT AT ASSIUT UNIVERSITY HOSPITALS, EGYPT

Mahmoud, Marwa M.; Hassanein, Khaled M.; Alshammari, Tahani Obaid; Batiha, Gaber El-Saber; Hetta, Helal F.

doi:10.21608/bfsa.2022.239583

DETECTION OF CARBAPENEM RESISTANCE AMONG ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE IN CHEST INTENSIVE CARE UNIT AT ASSIUT UNIVERSITY HOSPITALS, EGYPT

Document Type : Original Article

Authors

¹ Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, Egypt.

² Clinical laboratory, The College of Applied Medical Sciences, Aljouf University.

³ Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt.

10.21608/bfsa.2022.239583

Abstract

This study aimed to determine antimicrobial susceptibility pattern, percentage of carbapenem resistance and presence of blaNDM1, blaVIM1, blaIMP, blaKPC genes among Escherichia coli (E.coli) and Klebsiella pneumonia (K. pneumonia) isolated from chest intensive care unit (ICU) at Assiut University Hospitals, Egypt. Antibacterial susceptibility was detected by disc diffusion method. Genotypic detection of carbapenem resistant genes (IMP, NDM, VIM, and KPC) was done by PCR. From totally 200 patient clinical samples, 100 isolates (50%) were identified to be E.coli and K. pneumonia. Various percentages of resistance were reported for oxacillin (80 and 60%), ciprofloxacin (46 and 52%), amikacin (66 and 86%) amoxicillin & clavulanic acid (60 and 90%) lomefloxacin (22 and 18%) gentamicin (70 and 54%) chloramphenicol (54.4 and 66.7%), imipenem (90 and 84%), meropenem (80 and 72%) for E.coli and Klebsiella. The prevalence of KPC, NDM1, IMP, VIM genes was 74, 56, 30, 26 % for E.coli and NDM1,KPC, VIM1, IMP was 66, 64, 54, 50 % For K. pneumoniae. In conclusion, carbapenemases have essential role in antibiotic resistance of E. coli and K. pneumonia.

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