IN-VITRO AND IN-VIVO HYPOGLYCEMIC EFFICACY OF ROSA DAMASCENA PETALS EXTRACTS

Alsalti, Abed Almajid; Hasan, Nouma; Aldia, Dima

doi:10.21608/bfsa.2022.271532

IN-VITRO AND IN-VIVO HYPOGLYCEMIC EFFICACY OF ROSA DAMASCENA PETALS EXTRACTS

Document Type : Original Article

Authors

¹ Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tishreen University, Latakia, Syria

² Department of Analytical and Food chemistry, Faculty of Pharmacy, Tishreen University, Latakia, Syria

10.21608/bfsa.2022.271532

Abstract

Rosa damascena, which belongs to the Rosaceae family, is considered as one of the most important plants used in traditional medicine due to its different pharmacological properties, such as treatment of abdominal and chest pain, menstrual bleeding, and reduction of inflammation. The objective of our study was to investigate the hypoglycemic efficacy of R. damascene.
Aqueous and methanolic extracts were prepared from the petals of R. damascena mill (RDM). The phenolic content of the extracts was assessed using the Folin-Ciocalteu colorimetric method. The hypoglycemic activity was evaluated by the determination of in vitro alpha-amylase inhibitory activity. The in vivo post-prandial hypoglycemic effect was performed in normal and alloxan-induced diabetic mice. Acarbose (alpha-glucosidase inhibitor) was used as a standard drug.
The total phenolic content of aqueous and methanolic extracts was 29.23 gGAE/l and 58.8 gGAE/l. The alpha-amylase inhibition assay was performed using concentrations of (20, 40, 60, 80, 100, and 120 µg/ml) of aqueous extract, methanolic extract, and acarbose. The percentage inhibition of alpha-amylase was dependent on dose and varied between (33.66-89.96%), (41.57-92.16%) and (23.4-81.82%), respectively. Both aqueous and methanolic extracts revealed higher inhibitory activity of alpha-amylase compared to acarbose.
Oral administration of methanolic extract in normal and diabetic mice significantly decreased glucose blood levels after maltose loading in a dose-dependent manner.
These results suggest that R. damascena petal extracts might have a hypoglycemic effect. They also imply that R. damascena may have anti-diabetic properties by inhibiting of alpha-glucosidase-mediated carbohydrate absorption in the intestine and lowering postprandial glucose levels.

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