ASSOCIATION BETWEEN ANTIBIOTIC RESISTANCE, BIOFILM FORMATION AND LASB GENE IN PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT CLINICAL SPECIMENS

Document Type : Original Article

Authors

1 Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt

2 Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt / Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia 11566, Egypt

Abstract

A microorganism's capacity to form a biofilm is seen as a sign of a clinically significant infection. Pseudomonas aeruginosa infections are challenging to treat since the majority of isolates have high levels of innate resistance to numerous antibiotics and a propensity to develop biofilms. A total of 350 specimens were collected from patients. 125 isolates of P. aeruginosa were recovered from different clinical samples. The antibiotic susceptibility of these isolates against Ciprofloxacin, Amikacin, cefepime, Norfloxacin, imipenem, Genamicin, Tobramycin, Aztreonam, Piperacillin-tazobactam and Colistin was determined using disk diffusion method.  The TCP technique assay was chosen to identify the development of biofilm. Elastase gene detection was carried out using polymerase chain reaction (PCR) (lasB). The antibiotics against which there was the greatest resistance were cefepime (92.8%) and ciprofloxacin (67.2%). TCP technique identified 15 as weak or non-biofilm producers, 32 as moderate, and 78 as robust producers of biofilm. In 89.6% of P. aeruginosa isolates, LasB was found. P. aeruginosa's pattern of antibiotic resistance was more prevalent in biofilm producers than in non-producers. We come to the conclusion that P. aeruginosa biofilm development and drug resistance have a positive association.

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