OBESITY IS ASSOCIATED WITH AUTOPHAGY DYSREGULATION IN EGYPTIAN WOMEN

Saleem, Tahia; M Salama, Ragaa H; Mohamed, Ghada A; Qawy, Abdel Rahman H Abdel; Radwan, Eman

doi:10.21608/bfsa.2023.301190

OBESITY IS ASSOCIATED WITH AUTOPHAGY DYSREGULATION IN EGYPTIAN WOMEN

Document Type : Original Article

Authors

¹ Department of Medical Biochemistry, Faculty of Medicine, Assiut university, Assiut, Egypt

² Department of Internal Medicine, Faculty of Medicine,Assiut university, Assiut, Egypt

³ Department of Biochemistry, faculty of pharmacy, Assiut university, Assiut, Egypt

⁴ Department of Medical Biochemistry, Faculty of Medicine, Assiut university, Assiut, Egypt/ Department of Biochemistry, Sphinx University, New Assiut City, Assiut, Egypt

10.21608/bfsa.2023.301190

Abstract

Background: Obesity causes a reduced life quality and economic burden to Egyptians. Therefore, it is of utmost importance to investigate its risk factors to establish potential preventive strategies for it and its future complications. Obesity and its associated stress insults can often interfere with the autophagic process through various mechanisms, which can result in further aggravation of obesity-related metabolic pathologies. This case control study aimed at investigating autophagy dysregulation in Egyptian obese women. Methods: This study comprised 85 women that were classified into 5 groups (17 persons each):1- healthy, 2-overweight, 3-moderate obese, 4-severe obese and 5-morbid obese group. The expression of autophagy related gene (ATG5) was estimated by qRT-PCR, whereas, the serum activity of P70S6 kinase was assayed by ELISA. Results: The data revealed elevated mRNA expression levels of ATG5 with a significantly high serum P70s 6 kinase-1 in a stepwise fashion in obese subgroups. Conclusion: The present study demonstrated increased levels of both autophagy parameters in obese women that mean autophagy dysregulation. ROC curve analysis showed that p70S6 kinase-1 is a useful biomarker for both diagnosis and prognosis of obese subgroups. On the other hand, ATG5 was able to discriminate between the severe and moderate obese women. These data may represent a possible protective value for obese women, in addition to establishing potential strategies for autophagy inhibition in obesity and in turn, alleviation of its future complications.

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