IN VIVO WOUND HEALING AND IN VITRO ANTI- ANTIOXIDANT ACTIVITY EVALUATION OF COTULA CINEREA

LAKACHE, Zineb; Sadallah, Mohcene; Aliboudhar, Hamza; HACIB, Hinda; Abdelkrim, Kameli; TOUNSSI, Hassina

doi:10.21608/bfsa.2023.220458.1798

IN VIVO WOUND HEALING AND IN VITRO ANTI- ANTIOXIDANT ACTIVITY EVALUATION OF COTULA CINEREA

Document Type : Original Article

Authors

¹ Laboratory of Ethnobotany and Natural Substances, ENS-Kouba, Algiers, Algeria

² ENS kouba

³ USTHB, Laboratory of Functional Organic Analysis, Faculty of Chemistry, University of Sciences and Technology Houari Boumediene, El Bab-Ezzouar, Algiers, Algeria

⁴ Laboratory of Ethnobotanyand Natural Substances, ENS-Kouba, Algiers

10.21608/bfsa.2023.220458.1798

Abstract

The objective of this research was to identify the primary constituents of the essential oil obtained from Cotula cinerea (Delile) Vis. through the process of hydrodistillation. Additionally, the study aimed to explore the oil's in vivo wound healing properties and its in vitro antioxidant capabilities. Several compounds were identified in the essential oil, including thujone, santolina triene, camphor, and 1,8-cineole. To assess its wound healing potential, the essential oil was topically applied to induced wounds in mice, resulting in a notable acceleration of wound healing and repair, with a reported rate of 93.2 ± 0.7%. Furthermore, the research included an evaluation of the essential oil's analgesic activity, which was assessed by administering intraperitoneal injections of acetic acid to mice. Moreover, the antioxidant activity of the essential oil was assessed through the ferric reduction antioxidant power and DPPH scavenging assay. The findings from these antioxidant tests revealed that the essential oil extracted from Cotula cinerea exhibited the ability to reduce iron and scavenge DPPH radicals effectively. These results underscore the bioactive characteristics of Cotula cinerea essential oil and its potential for synergistic effects. Consequently, Cotula cinerea shows promise as a natural source of biologically active compounds with therapeutic potential for wound healing. These discoveries suggest that Cotula cinerea could serve as a viable alternative to synthetic wound treatment medications in the future pharmaceutical industry.

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