ANTIMICROBIAL AND IN VITRO CYTOTOXICITY EFFECT OF GREEN SYNTHESIZED SILVER NANOPARTICLES USING AQUEOUS LEAF EXTRACT OF SENNA HIRSUTA

Palei, Narahari N; Bhuvana, M. P.; Jayaraman, R.; Dhar, Arghya Kusum

doi:10.21608/bfsa.2023.327539

ANTIMICROBIAL AND IN VITRO CYTOTOXICITY EFFECT OF GREEN SYNTHESIZED SILVER NANOPARTICLES USING AQUEOUS LEAF EXTRACT OF SENNA HIRSUTA

Document Type : Original Article

Authors

¹ Amity Institute of Pharmacy, Amity University Uttar Pradesh, Lucknow Campus, India

² Sree Vidyanikethan College of Pharmacy, Tirupati, Andhra Pradesh, India

³ Shri Venkateshwara College of Pharmacy, Ariyur, Pondicherry, India

⁴ School of Pharmacy, The Neotia University, Sarisha, West Bengal, India

10.21608/bfsa.2023.327539

Abstract

In this study, we prepared silver nanoparticles (AgNPs) using an aqueous Senna hirsuta leaf extract. The AgNPs were characterized using UV visible spectrophotometer, particle size, zeta potential, energy diffraction X-ray (EDAX), Fourier transform infrared (FTIR), transmission electron microscopy (TEM) and determined antibacterial and cytotoxicity impact of the AgNPs. UV-Visible spectroscopy of Senna hirsuta leaf extract synthesized into AgNPs was carried out at various time intervals. The peak (λ max) was observed at 436–446 nm and it was broad. The particle size of the synthesized AgNPs was found 28.3 nm. The Zeta potential of AgNPs was found -4.4 mV. TEM analysis demonstrated that nanoparticles have a spherical form. According to the EDAX analysis, the extract effectively capped the AgNPs. Zone of inhibition of AgNPs were observed more than those of the extract. Silver nanoparticles demonstrated effective antimicrobial activity due to their large surface area, which allows for stronger contact with cell wall of bacteria. In vitro cytotoxicity showed that synthesized AgNPs a prominent cytotoxicity activity against MCF7 cell lines

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