Document Type : Original Article
Authors
1
Nutrition and Food Science, Faculty of Specific Education, Tanta University, Egypt
2
Zoology Department, Faculty of Science, Tanta University, Egypt
3
Biochemistry Division, Chemistry Department, Faculty of Science, Tanta University, Egypt
4
Zoology Department, Faculty of Science, Damanhur University, Egypt
Abstract
Cyclophosphamide (CTX) is a well-known broad-spectrum anticancer agent however; it has severe side effects on vital organs. Portulaca oleracea that belongs to family Portulacaceae showed promising nutritional and biomedical properties. This research aimed to estimate the effect of P. oleracea leaves extract (POLE) on CTX toxicity in male CD-1 mice. Total phenolic, flavonoids contents, total antioxidant capacity (TAC), and DPPH scavenging activity (DPPH %) were determined in POLE by phytochemical quantitative analysis. Thirty-six male CD-1 mice were divided into three groups (n=12), as negative control, CTX (200 mg/kg) injected, and CTX/POLE (200 mg/kg) injected groups. Blood samples, liver tissues and kidney tissues were collected for hematological, biochemical and histopathological investigations. The total phenolic, flavonoid content, saponin, DPPH %, and total antioxidant capacity (TAC) were 1368 µg/ml, 302 µg/ml, 0.1250 µg/g, 65%, 193 µg/ml, respectively. Treatment with POLE augmented the hematological, biochemical, and histopathological changes induced by CTX of hepatic and renal tissues. Collectively, POLE reduced the hepato-renal toxicities caused by CTX in mice by improving the antioxidants/oxidants hemostasis
Keywords
)
View on SCiNiTO