SPIRONOLACTONE ALTERS THE LEVELS OF NEURONAL FUNCTION MARKERS IN AUTISTIC RATS

Mirza, Roohi; Sharma, Poonam; Kulkarni, G T.; Sharma, Bhupesh

doi:10.21608/bfsa.2023.327619

SPIRONOLACTONE ALTERS THE LEVELS OF NEURONAL FUNCTION MARKERS IN AUTISTIC RATS

Document Type : Original Article

Authors

¹ Department of Pharmacology, Amity Institute of Pharmacy, Amity University Uttar Pradesh, Noida, India

² Gokaraju Rangaraju College of Pharmacy, Hyderabad

10.21608/bfsa.2023.327619

Abstract

Autism spectrum disorder (ASD) is a neurodevelopmental disorder that is characterized by repetitive behavior and impairment in social behavior. ASD is a complex disorder with complex etiology that involves multiple genes, environmental factors, and epigenetic mechanism. Many clinical and pre-clinical study have demonstrated the association of propionic acid (PPA) with ASD. This study evaluates the potential effect of spironolactone in PPA induced ASD phenotype. PPA (250 mg/kg, po) was administered to Albino Wistar rats from post-natal day (PND) 21^st to 23^rd to induce ASD like neurobehavioral and neurobiochemical alterations. Animals were experimented for behavioral (elevated plus maze, three chambered social interaction apparatus, and Y-maze apparatus), biochemical parameters (BDNF, synapsin II), and blood-brain barrier impairment. Post-natal PPA exposure resulted impairment in social behavior, anxiety, and repetitive behavior in animals. Furthermore, PPA exposure caused reduction in the levels of BDNF and synapsin II in rats’ brain. Spironolactone (25 mg/kg and 50 mg/kg) administration was observed to ameliorate post-natal PPA exposed behavioral and biochemical impairments in animals.

Keywords