Chemical profile and membrane stabilization activity: unveiling the anti-inflammatory mechanism of myrrh

Document Type : Original Article

Authors

Medicinal Chemistry Department, Theodor Bilharz Research Institute. Kornish El-Nile St., Warrak El-Hadder, Imbaba, Mail Box 30, P.O. 12411, Giza, Egypt

Abstract

Commiphora myrrha has been extensively used in traditional medicine. The biochemical characters of Commiphora myrrha hydro-methanolic and petroleum ether extracts were studied. The phenolic and flavonoid contents, DPPH assay, in vitro anti-inflammation activity of them by determining the membrane stabilizing activity and NO assay as well as chemical profile by GC-MS and HPLC were investigated. The results showed that phenolic and flavonoid content of hydro-methanolic extract (158.26 ± 3.44 mg Gallic acid equivalent (GAE)/g of extract and 91.43 ± 3.33 Rutin /g extract, respectively was higher than those of petroleum ether (126.16 ± 0.42 mg GAE/g of extract and 61.97 ± 2.5 mg Rutin /g extract, respectively). Also, hydro-methanolic has potent antioxidant activity than petroleum ether (IC50 = 46.56 ± 1.06 and 66.13 ± 1.64 μg/mL, respectively). Moreover, hydro-methanolic extract exhibited strong stabilizing effects on human red blood cell membrane as well as strong NO production inhibition. HPLC for hydro-methanolic extract showed 13 phenolic compounds and 8 flavonoid one. While, GC-MS analysis exhibited 49 compounds in the petroleum ether extract and 45 ones in the hydro-hydro-methanolicic extract. In general, the anti-inflammatory effect is correlated to antioxidant activity of the plant depends on the phenolic and flavonoid content.

Keywords

Bulletin of Pharmaceutical Sciences Assiut University

Articles in Press, Accepted Manuscript
Available Online from 21 April 2024

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