GC-MS Analysis and Evaluation of Antioxidant, Anticancer, Cytotoxicity, and Hypoglycemic Properties of the Libyan Moringa oleifera Leaf Extract on Alloxan-Induced Diabetes in Mice

Document Type : Original Article

Authors

1 Assiut University Mycological Centre, Assiut University

2 The Advanced Center for Plant Research and Complementary Medicine, Zawia University; Zawia, Libya

3 Department of Biological Sciences, University of Arkansas, Fayetteville, AR 72701, USA

4 Department of Botany & Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

Abstract

Libyan Moringa oleifera leaves were extracted using methanol, and the bioactive components' antibacterial, antioxidant, anti-inflammatory, and anticancer characteristics were assessed using gas chromatography mass spectrometry analysis (GC-MS). Moringa oleifera exhibited antioxidant activity, with an IC50 of 11.53 µg/mL. Wi38 cell line's viability decreased with increasing M. oleifera extract concentration, while its cytotoxicity increased with an IC50 of 393.41±7.77 μg/mL. Alloxan-induced diabetes model in Swiss albino mice was implemented. Three different doses (100, 200, or 300 mg/kg of mice body weight) of M. oleifera’s extract were evaluated for their effects on blood sugar concentration and the histology of the pancreatic islets. The normoglycemic group did not experience hypoglycemia as a result of the extract intake. Blood glucose levels were noticeably lower at 200 and 300 mg/kg, especially with the 300 mg/kg dose. The 300 mg/kg dose considerably decreased the fasting blood sugar by the fourth week without endangering hypoglycemia. Giving 100 and 200 mg/kg of M. oleifera extract to the diabetic control group resulted in varied alloxan attack recovery and regeneration of islet and acin. The morphology and acini of the pancreatic islets underwent a significant modification following the ingestion of 300 mg/kg of M. oleifera extract. They were notably bigger and more revitalized, and the number of β-cells that adopted a spherical shape significantly increased.

Keywords

Bulletin of Pharmaceutical Sciences Assiut University

Articles in Press, Accepted Manuscript
Available Online from 23 May 2024

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