DYSREGULATION OF EZRIN EXPRESSION AND PHOSPHORYLATION IN UTERINE LEIOMYOMA

Document Type : Original Article

Authors

1 Department of Biochemistry, Faculty of Pharmacy, South Valley University, Qena, Egypt

2 Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Egypt

3 Department of Biochemistry, Faculty of Pharmacy, Sphinx University, New Assiut, Egypt

4 Medical student, Faculty of Medicine, Assiut university, Egypt

Abstract

Uterine Leiomyoma (UL) is a major source of gynecologic and reproductive dysfunction in females of reproductive age. They are highly fibrotic tumors with evident disturbance of the mechano-transduction molecules. Ezrin acts as a dynamic linkage between the cytoskeleton and the membrane-associated proteins known to be dysregulated in tumorigenesis. Aim of the study: The current study aimed to profile the expression of ezrin, phospho-ezrin, and their upstream effector molecules; RhoA and ROCK1 in UL, their adjacent tissues, and control myometrium. Materials and Methods: Ezrin, phospho-ezrin, RhoA and ROCK1 mRNA and proteins were assayed in the tissue lysate of fibroid, and adjacent myometrium of 43 pre-menopausal women with symptomatic UL and 18 non-fibroid myometrium as a control group. Results: Tissue levels of ezrin, phospho-ezrin, RhoA and Rock in fibroid and adjacent tissues were higher compared to the control tissue. Moreover, the levels of phospho-ezrin and total RhoA proteins showed higher levels in fibroid compared to adjacent tissues. Phospho-ezrin and ROCK1 mRNA correlated positively with both uterine and fibroid size. Conclusion: Ezrin, phospho-ezrin, and RhoA-ROCK upregulation in leiomyoma and its adjacent myometrium signify the central role of ezrin and its upstream and downstream effector on the pathogenesis and progression of UL.

Keywords

Bulletin of Pharmaceutical Sciences Assiut University
Volume 47, Issue 2
December 2024
Pages 1327-1339

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