PHENOLIC CONTENT, ANTIOXIDANT, AND ANTIBACTERIAL ACTIVITIES OF HYDROMETHANOLIC EXTRACT OF LIMNOSPIRA FUSIFORMIS TL03 ISOLATED FROM TELAMINE LAKE, NORTHWEST ALGERIA

Belkacem, Guenachi; Hakima, Korteby  Mefti; Lynda, Lamari

doi:10.21608/bfsa.2024.293271.2143

PHENOLIC CONTENT, ANTIOXIDANT, AND ANTIBACTERIAL ACTIVITIES OF HYDROMETHANOLIC EXTRACT OF LIMNOSPIRA FUSIFORMIS TL03 ISOLATED FROM TELAMINE LAKE, NORTHWEST ALGERIA

Document Type : Original Article

Authors

¹ Blida 1 University, Laboratory of Aromatic and Medicinal Plants Research, Natural Sciences and Life Faculty, Blida, Algeria

² National Center for Research and Development of Fisheries and Aquaculture (CNRDPA), Bou Ismail, Algeria

³ Biology Laboratory of Microbial Systems, Higher Normal School of Kouba, Algiers, Algeria

10.21608/bfsa.2024.293271.2143

Abstract

This study was conducted to evaluate the total phenolic, flavonoid content, antioxidant, and antibacterial activity of hydomethanolic Limnospira fusiformis TL03 .

The total phenolics and flavonoids were determined by using the Folin-Ciocalteu and aluminum chloride methods, respectively. The YL 9100 HPLC was used to identify the phenolic compounds. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and beta-carotene-linoleic acid tests were used to evaluate antioxidant activity in vitro. The antimicrobial activities were tested against six bacteria using the disc diffusion method and the agar dilution method.

The total phenolic and flavonoids contained in the hydromethanolic extract were 18.41 ±0.18 mg GAE/g DW and 2.11 ±0.12 mg quercetin/g DW, respectively. The polyphenol analysis showed the presence of: Ascorbic acid, Gallic acid, Luteotin , Quercetine, Kaempferol.

This extract shows strong DPPH radical scavenging and β-carotene bleaching activities with a percentage of 86.19±1.61% and 85.87±1.65%, respectively, in addition to activity antibacterial, in which the zone inhibition diameters (ZID) range from 33.33±1.53 to 11±1 mm, with the higher value antibacterial activity (33.33±1.53 mm) registered against S. aureus and the lower minimum inhibitory concentrations (MIC) observed against K pneumonia.

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