DEVELOPMENT OF AYURVEDIC FORMULATIONS FROM LEAVES OF CARICA PAPAYA AND COMPARISON OF PLATELET AUGMENTATION POTENTIAL AGAINST CYCLOPHOSPHAMIDE-INDUCED THROMBOCYTOPENIA

Jyothi, Divya; Joshi, Himanshu; Dsouza, Ullas Prakash; Rizwana, Kadeejath

doi:10.21608/bfsa.2024.295738.2151

DEVELOPMENT OF AYURVEDIC FORMULATIONS FROM LEAVES OF CARICA PAPAYA AND COMPARISON OF PLATELET AUGMENTATION POTENTIAL AGAINST CYCLOPHOSPHAMIDE-INDUCED THROMBOCYTOPENIA

Document Type : Original Article

Authors

¹ Department of Pharmacognosy, Pharmacy, Nitte deemed to be University, NGSM Institute of Pharmaceutical Sciences, Mangalore, India

² Pharmacology Department,Graphic Era Hill University, Bhimtal, Uttarakhand, India

³ Department of Pharmacology, NGSM Institute of Pharmaceutical Sciences Institute of Pharmaceutical Sciences, Nitte (Deemed to be) University, Mangalore, India

⁴ NGSM Institute of Pharmaceutical Sciences, Nitte (Deemed to be) University, Deralakatte, Karnataka, India

10.21608/bfsa.2024.295738.2151

Abstract

Present study is aimed to prepare swarasa and ghrita formulations from Carica papaya leaves and compare the platelet-boosting effect of these formulations against the cyclophosphamide-induced thrombocytopenia model. Swarasa was obtained by crushing the leaves in blender followed by filtration. Ghrita was prepared by combining the fine paste of leaf (Kalka), molten cow ghee (Sneha Dravya), Drava Dravya in a specified ratio of 1:4:16 respectively and boiled till the complete evaporation of moisture. The organoleptic characters and physicochemical characteristics of ghrita were evaluated as per the WHO guidelines, Ayurvedic Pharmacopoeia, and Indian Pharmacopoeia. Platelet augmentation activity of swarasa, ghrita and tablet (standard) formulations of Carica papaya leaf was evaluated in rats by inducing thrombocytopenia with Cyclophosphamide. On induction of thrombocytopenia by cyclophosphamide, platelet count, total WBC count, RBC count and haemoglobin content reduced, while bleeding time and clotting time increased which indicates thrombocytopenia. After treatment with formulations of Carica papaya, platelet count, total WBC count, RBC count and haemoglobin content increased, while bleeding and clotting time decreased. A significant effect was observed with ghrita formulation, with no side effects such as diarrhoea, ulcers. Results of platelet augmentation activity investigation indicates that the ghrita formulation was found to be the most efficacious, with better patient compliance and no side effects in comparison with swarasa and tablet formulations.

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