ENHANCEMENT OF THE EFFICIENCY OF RHODOTORULA MUCILAGINOSA MH341115 (AUMC13570) IN BIODEGRADATION OF MALACHITE GREEN TOXIC DYE FROM POLLUTED WATER

Document Type : Original Article

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Botany & Microbiology Department, Faculty of Science, Assiut University

Abstract

Abstract

Malachite green (MG) dye is a widespread environmental contaminant that endangers human health and the stability of the Earth's biosphere. This research evaluated how environmental and cultural conditions influence the decolorization of malachite green by Rhodotorula mucilaginosa AUMC13570. A single factor was changed for every trial while maintaining the previously optimized variable constant in the one-step optimization method used for process optimization considering six main variables: incubation type (static and submerged), media composition (A, B, and C), temperature (25, 28, 30, and 37℃), agitation speed (50, 100, 120, and 150 rpm), inoculum age (one, two, three, four, five, and six) days, and initial dye concentration (50, 100, 200, 300, and 1000 mg/L). The decolorization capability was assessed by observing the reduction in dye absorbance every 2 hours during the 12-hour incubation period. Our findings showed that a dye concentration of 50 mg/L, incubation with agitation, a four-day inoculum age, an agitation speed of 150 rpm, and a temperature of 37℃ produced the highest decolorization rate. An analysis of the data highlighted various factors that influenced the decolorization effectiveness (p < 0.001).

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