IN VITRO ANTIMICROBIAL PHOTODYNAMIC ACTIVITY OF TECOMA STANS YELLOW FLOWERS EXTRACT LOADED IN NANO-EMULSION

Document Type : Original Article

Authors

1 Department of Medical Applications of Laser, National Institute of Laser Enhanced Sciences (NILES), Cairo University, Egypt

2 Pharmacognosy Department, National Research Centre, Dokki, Giza, Egypt

3 Department of medical applications of laser, National Institute of Laser Enhanced Sciences, Cairo University

4 Department of medical laser applications, The national Institute of Laser Enhanced Sciences, cairo university.

Abstract

Antimicrobial photodynamic therapy (aPDT) is an approach that was recently used to combat the resistance developed by pathogens toward antimicrobial drugs. Tecoma stans (L.) Jus., a prevalent tree in Egypt, was reported to be a promising photosensitizer in PDT. This study investigates the antimicrobial photodynamic activity of the methanolic crude extract of Tecoma stans (L.) Juss. ex Kunth (T. stans) loaded in a nano-emulsion as a natural photosensitizer. Molecular docking and molecular dynamics studies were conducted to investigate the binding between delphinidin as a promising lead and DNA's active site and to assess its stability through simulation The nano-emulsion formulation (TSFE-NE) was prepared, characterized, and evaluated for its antimicrobial photodynamic activities against three types of microorganisms: Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Staphylococcus aureus), and the pathogenic yeast Candida albicans. The percentage of microbial eradication was calculated after irradiation at 90 Mw/cm2. The microbial eradication of TSFE-NE was enhanced after light exposure. Eradication percentages of 97.43%, 90.56%, and 87.77% were obtained for Escherichia coli, Staphylococcus aureus, and Candida albicans, respectively. These results confirmed the efficacy of natural photosensitizer and nanotechnology combination for improving antimicrobial photodynamic activities

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