PHENOLIC COMPOUNDS, ANTIOXIDANT, AND ANTIMICROBIAL ACTIVITIES OF METHANOLIC EXTRACT FROM THE SAHARAN ENDEMIC PLANT HALOXYLON SCOPARIUM POMEL

Ahmed-Gaid, Kelthoum; Ahmed-Gaid, Nour El Yakine; Hanoun, Saida; Chenna, Houssem; Ghenaiet, Khaoula; Ahmed-Gaid, Hadjer; Hadef, Youcef

doi:10.21608/bfsa.2025.355360.2418

PHENOLIC COMPOUNDS, ANTIOXIDANT, AND ANTIMICROBIAL ACTIVITIES OF METHANOLIC EXTRACT FROM THE SAHARAN ENDEMIC PLANT HALOXYLON SCOPARIUM POMEL

Document Type : Original Article

Authors

¹ Department of Biological and Agricultural Sciences, Institute of Sciences and Technology, AbdelHafid Boussouf University Center, 43000, Mila, Algeria

² Pharmacognosy Laboratory, Department of Pharmacy, Faculty of Medicine, University of Badji Mokhtar, 23000, Annaba, Algeria

³ Department of Molecular and Cellular Biology, Faculty of Natural and life Sciences, University of Abbes Laghrour, 40004, Khenchela, Algeria Applied Molecular and Cellular Biology, Faculty of Natural and life Sciences, University of

⁴ Pharmaceutical Sciences Research Center (CRSP), Constantine, 25000, Algeria

⁵ Laboratory of Biophysics, Faculty of Medicine, University of Badji-Mokhtar, 23000, Annaba, Algeria

⁶ Public Health Institute of Mohamed Layachi, 8000, Bechar, Algeria Department of Medicine, Faculty of Medicine, University of Badji Mokhtar, 23000, Annaba, Algeria

⁷ Pharmacy Analysis Departemnt, Medecine Faculty, 23000, Annaba, Algeria.

10.21608/bfsa.2025.355360.2418

Abstract

This study aims to investigate the phenolic profile, antioxidant, and antibacterial activities of the methanolic extract of Haloxylon scoparium Pomel, an endemic plant of the Algerian Sahara. Quantitative analysis revealed total phenolic content of 54.77 mg GAE/g DM, flavonoids at 9.39 mg CE/g DM, and total tannins at 5.36 mg TAE/g DM. High-Performance Liquid Chromatography identified several phenolic compounds, including chlorogenic acid, caffeic acid, quercetin, catechin, rutin, and kaempferol. Antioxidant activity, assessed using the DPPH radical scavenging assay, demonstrated a strong activity with an IC50 value of 68.64 µg/ml. The methanolic extract exhibited significant antibacterial activity against various pathogenic bacteria, including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae, with inhibition zones ranging from 19.00 to 22.10 mm. The extract also showed promising activity against two multidrug-resistant strains. These findings support the potential use of H. scoparium as a valuable source of bioactive compounds, offering significant antibacterial properties that could be further explored for the development of alternative therapeutic agents.

Keywords