NARINGIN PREVENT CISPLATIN-INDUCED NEPHROTOXICITY BY ABROGATION OF OXIDATIVE STRESS AND INFLAMMATION IN RATS

Taha, Abd-Elmoniem A.; Khalifa, M. M.A.; Abd El-Salam, Mohamed I.

doi:10.21608/bfsa.2018.62467

NARINGIN PREVENT CISPLATIN-INDUCED NEPHROTOXICITY BY ABROGATION OF OXIDATIVE STRESS AND INFLAMMATION IN RATS

Document Type : Original Article

Authors

¹ Department of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University, Assiut, Egypt

² Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Egypt

10.21608/bfsa.2018.62467

Abstract

Nephrotoxicity remains one of the most dangerous effect relevant to cisplatin use in chemotherapy. Rat injection with cisplatin in a single dose of 7 mg/kg intraperitoneally resulted in a significant increase in serum level of urea and creatinine. Also, cisplatin caused marked increase in renal content of malondialdehyde (MDA), while depletion in reduced glutathione (GSH). In addition, cisplatin administration notably increased kidney/body weight ratio, renal contents of nitric oxide (NO), tumor necrosis factor alpha(TNF-α) and cyclooxygenase-2 (COX-2) protein level as well as renal myeloperoxidase (MPO) activity. Histopathological examination confirmed the biochemical and molecular results which revealed several pathological alteration in the renal tissues following cisplatin. Oral pretreatment of rats with naringin (NAR) (80 mg/kg) for 14 days before and 7 days after cisplatin injection significantly reduced the pathological level of serum urea and creatinine and restored oxidative stress parameters. In the same manner, the inflammatory markers as well as kidney/body weight ratio show great improvement following the treatment. The histopathological examination confirms fit with the biochemical and molecular results. In conclusion, NAR showed a great protective effect against cisplatin-induced nephrotoxicity in rats via its antioxidant, anti-inflammatory roles.