ESTABLISHMENT OF CALLUS AND CELL SUSPENSION CULTURES OF CASSIA BICAPSULARIS L.

Document Type : Original Article

Authors

1 Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

2 Institute of Pharmaceutical Biology, Technische Universität Braunschweig, Mendelssohnstrasse 1, 38106 Braunschweig, Germany

Abstract

Callus cultures were initiated from leaf of Cassia bicapsularis L. on solid Murashige &
Skoog (MS) basal medium supplemented with different growth regulators. Excellent growth of
callus was obtained in medium supplemented with 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-
D) and grown in the dark. The obtained callus was subcultured every 4 weeks in the dark at
25°C. The Callus was compact, yellowish brown in colour and used for establishment of cell
suspension cultures. Maximum growth of suspension cultures was achieved in medium
supplemented with 1 mg/l 2,4-D and 0.1 mg/l kinetin. The growth rate of cells was initially slow
but as the cultures proceeded, the growth increased significantly over a period of 22 days then
the growth of cells was stable for 35 days.

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