From the methanolic extract of fresh fruits of Solanum reflexum Schrank, a major compound was isolated and identified as; aculeatiside-A 1. The structure of the isolated compound was elucidated on the basis of 1H and 13C and DEPT-13C-NMR; in addition to comparison with reported data. The cytotoxic effect of aculeatiside-A on hepatocellular carcinoma cells and its mechanism was investigated. It was accomplished that aculeatiside-A inhibit the growth of HepG-2 cells significantly by induction of apoptosis. Marked morphological changes of apoptosis were observed clearly. The results for the first time demonstrate that aculeatiside-A has significant anti-proliferation effect by induction of apoptosis on hepatocellular carcinoma cells in-vitro, down regulation of Survivin and Bcl-2 expressions as well as up regulation of Bax and P53 expression may be one of the important apoptotic inducing mechanisms.
Nafady, A., & Mohafez, O. (2011). CYTOTOXICITY OF ACULEATISIDE-A TO HEPATOCELLULAR CARCINOMA CELLS DETERMINED BY MTT BIOASSAY. Bulletin of Pharmaceutical Sciences Assiut University, 34(2), 121-129. doi: 10.21608/bfsa.2011.63257
MLA
Alaa Mohamed Nafady; Omar Mahmoud Mohamed Mohafez. "CYTOTOXICITY OF ACULEATISIDE-A TO HEPATOCELLULAR CARCINOMA CELLS DETERMINED BY MTT BIOASSAY". Bulletin of Pharmaceutical Sciences Assiut University, 34, 2, 2011, 121-129. doi: 10.21608/bfsa.2011.63257
HARVARD
Nafady, A., Mohafez, O. (2011). 'CYTOTOXICITY OF ACULEATISIDE-A TO HEPATOCELLULAR CARCINOMA CELLS DETERMINED BY MTT BIOASSAY', Bulletin of Pharmaceutical Sciences Assiut University, 34(2), pp. 121-129. doi: 10.21608/bfsa.2011.63257
VANCOUVER
Nafady, A., Mohafez, O. CYTOTOXICITY OF ACULEATISIDE-A TO HEPATOCELLULAR CARCINOMA CELLS DETERMINED BY MTT BIOASSAY. Bulletin of Pharmaceutical Sciences Assiut University, 2011; 34(2): 121-129. doi: 10.21608/bfsa.2011.63257
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