SPECTROFLUORIMETRIC METHOD FOR DETERMINATION OF SOME OXICAMS USING POTASSIUM BROMATE

A sensitive and selective spectrofluorimetric method has been
developed for the determination of some non-steroidal antiinflammatory
oxicams derivatives namely: tenoxicam (Tx),
piroxicam (Px) and lornoxicam (Lx) after their complete oxidative
acidic hydrolysis to 2-aminopyridine. The hydrolytic product 2-
aminopyridine exhibits fluorescence emission at 365 nm (excitation
at 305 nm). The optimal conditions of the reaction were
investigated. The method was found to be linear in the ranges of
(0.015-0.500 μg/ml) for Tx (0.006-0.300 μg/ml) for Px and (0.060-
0.200 μg/ml) for Lx. The suggested method was successively
applied for the determination of the studied drugs in different
dosage forms with a recovery percentages ranged 96.82-102.79 ±
0.614-2.578. The method was also applied for the determination of
the drugs in spiked urine with a recovery percentages ranged
80.51-105.35 ± 1.067-5.338. The validity of the method was assessed according to USP guidelines. Statistical analysis of the
results reveled high accuracy and good precision.