SPECTROPHOTOMETRIC AND SPECTROFLUORIMETRIC DETERMINATION OF 1,4-DIHYDROPYRIDINE DRUGS USING POTASSIUM PERMANGANATE AND CERIUM (IV) AMMONIUM SULPHATE

Document Type : Original Article

Authors

1 Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

2 Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia 61519, Egypt

3 Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, Egypt

Abstract

Simple and sensitive spectrophotometric and spectrofluorimetric methods have been
developed for determination of 1,4-dihydropyridine (1,4-DHP) drugs based on the oxidation of
the investigated 1,4-DHP drugs with acidic KMnO4 (method I) or Ce (IV) (method II). The first
method is based on the decrease in the colour of the permanganate solution due to the presence
of the studied drug was measured at 525 nm. And the second method is based on monitoring the
fluorescence of the produced cerium (III) at emission 355 nm (excitation at 255 nm). All
variables that affect the performance of the proposed methods were carefully studied and
optimized. The analytical performance of the methods was validated according to International
Conference of Harmonization guidelines. The proposed methods were applied successfully to
the determination of the drugs in commercial tablets and capsules. The results of the proposed
procedures were statistically and compared with those obtained by the reference methods.

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