A simple and sensitive reversed phase high-performance liquid chromatographic method was developed for simultaneous quantification of diltiazem HCl and its major metabolite Ndemethyldiltiazem in human plasma. The method involves one step solvent extraction of diltiazem, N-demethyldiltiazem and the internal standard, verapamil with n-hexane and diethyl ether (50:50 v/v). The mobile phase comprised 0.1 M ammonium dihydrogen phosphate-acetonitrile (62:38 v/v) and triethylamine (0.08%) was added before the pH was adjusted to 5.9 with 85% phosphoric acid. Analysis was run at a flow rate of 1.0 ml/min at a detection wavelength of 238 nm. The completion time for assay was not more than 10 minutes and lower limit of quantification was 5 ng/ml. The calibration curve for diltiazem and its metabolite was linear over a concentration range of 5-200 ng/ml and average recovery was about 90%. The coefficient of variation and percent error values of the assay method within and between days were all less than 10%.
Hasan, S., Rahman, N., Hussain, K., & Raza, S. (2006). SIMULTANEOUS HPLC-UV QUANTIFICATION OF DILTIAZEM AND N-DEMETHYLDILTIAZEM IN HUMAN PLASMA. Bulletin of Pharmaceutical Sciences Assiut University, 29(1), 1-8. doi: 10.21608/bfsa.2006.64822
MLA
S. S. Hasan; Nisar ur Rahman; Khalid Hussain; S. Atif Raza. "SIMULTANEOUS HPLC-UV QUANTIFICATION OF DILTIAZEM AND N-DEMETHYLDILTIAZEM IN HUMAN PLASMA". Bulletin of Pharmaceutical Sciences Assiut University, 29, 1, 2006, 1-8. doi: 10.21608/bfsa.2006.64822
HARVARD
Hasan, S., Rahman, N., Hussain, K., Raza, S. (2006). 'SIMULTANEOUS HPLC-UV QUANTIFICATION OF DILTIAZEM AND N-DEMETHYLDILTIAZEM IN HUMAN PLASMA', Bulletin of Pharmaceutical Sciences Assiut University, 29(1), pp. 1-8. doi: 10.21608/bfsa.2006.64822
VANCOUVER
Hasan, S., Rahman, N., Hussain, K., Raza, S. SIMULTANEOUS HPLC-UV QUANTIFICATION OF DILTIAZEM AND N-DEMETHYLDILTIAZEM IN HUMAN PLASMA. Bulletin of Pharmaceutical Sciences Assiut University, 2006; 29(1): 1-8. doi: 10.21608/bfsa.2006.64822