UTILITY OF 5-DIAZO-1,2,4-TRIAZOL-3-CARBOXYLIC ACID FOR COLORIMETRIC DETERMINATION OF SOME DIBENZAZEPINES

Document Type : Original Article

Authors

1 Department of Pharmaceutical Medicinal Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt

2 Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt

Abstract

A simple, rapid, and accurate spectrophotometric method for determination of four dibenzazepines has been developed. The method is based on the interaction of 5-diazo-1,2,4-triazole-3-carbaxylic acid (DTCA) with dibenzazepine drugs in 10% nitric acid media. The resulting colored products possess 2 absorption maxima, one in the range of 475-495 nm with higher molar absorptivity and the other in the range of 570-575 nm with lower molar absorptivity. Measurements were performed in the range of 475-495 nm. Beer's law is obeyed in the concentration range of 2-10 µg/ml for both imipramine HCl and desipramine HCl and 2-14 µg/ml for both clomipramine HCl and trimipramine maleate. The molar absorptivities of the studied drugs are 2.877x 104 for imipramine HCl at 487 nm., 3.164 x 104 for desipramine HCl at 495 nm., 2.002 x l04 for clomipramine HCl at 475 nm. and 2.350 x 104 for trimipramine maleate at 475 nm. The proposed method is applied successfully for the determination of studied drugs in pure form and in commercial and laboratory prepared tablets. The obtained results are in good agreement with those obtained from official methods.

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