INFLUENCE OF -CYCLODEXTRIN ON THE NYSTATIN RELEASE FROM OINTMENTS AND ITS ANTIFUNGAL EFFECT

Document Type : Original Article

Authors

1 Department of Pharmaceutics, Faculty of Pharmacy, Assiut University, Assiut, Egypt

2 Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt

3 Department of Microbiology, Faculty of Medicine, Assiut University, Assiut, Egypt

Abstract

Thesolid complex of nystatin-g-cyclodextrin (g-CD) in a molar ratio 1:1 was prepared. Physico-chemical characteristics of the nystatin-g-CD) complex was studied by x-ray diffraction, differential scanning calorimetry and infrared spectroscopy. Absorption and hydrophilic (o/w) ointments were prepared, containing each of the intact drug, the g-CD complex and .the physical mixture of the drug with g-CD; respectively.
The in-vitro release of nystatin from both types of ointments was examined and the concentration of nystatin released was estimated by HPLC method using Cyclobond column-for the resolution of the drug. The release kinetic mechanism was found to follow both diffusion controlled and first-order kinetic mechanisms. The solid complex-prepared showed improved release from o/w rather than absorption ointment bases as well as enhanced antifungal effect against Candida Albicans.

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