EFFECT OF CERTAIN MACROMOLECULES ON THE EXTRACTION OF SOPHORA ALKALOIDS

Document Type : Original Article

Authors

1 Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Assiut, Egypt

2 Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt

Abstract

Sophora matrine alkaloids have recently been useful in the treatment of some types of cancer.
Certain classes of macromolecules including non-ionic surfactants, polyethylene glycols and cyclodextrins have been investigated for their effects on the extraction of Sophora alkaloids from the,leaf, the bark and the seed.
Increasing the non-ionic surfactant solutions above CMC improved the yield of the extracted alkaloids calculated and quantified after TLC separation as cytisine. Extending the hydrocarbon chain in a homologous series of non-ionic surfactants containing the same polyoxyethylene chain improved the extraction from the leaf, the bark and the seed and vice versa. That is why polysorbate 80 is more efficient than ploysorbate 20 and Brij 58 is more efficient than Brij 35. On the otherhand Eumulgin C 1500 is less efficient than Eumulgin C 1000 and Myrj 59 is less efficient than Myrj 53.
PEGs improved the extraction of Sophora leaf alkaloids irrespective of the concentration. Increasing the molecular weight of PEGs leads to decrease in the yield of cytosine extracted from the leaf.
Cyclodextrins improved the yield of cytisine extracted from Sophora leaf. It was found that b-cyclodextrin is more effective than a-cyclodextrin in this concern. In both cases the inclusion isotherm was found to be AN  type with no precipitation of the inclusion compound.

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